FGSC #7361

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Reporting Genes: Not available; tolC trp-4;lys(122)

Species: tetrasperma

Allele: C Y2198;122

Alternate Strain Number: DJ471-15a

Depositor: DJJ

Linkage Group: IVR R:I

Mating Type: a

Species Number: 12

ref1: Jacobson 1992 Genome 35:347-353, https://doi.org/10.1139/g92-053

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
trp-4	IVR. Between his-5 (3 to 7%) and leu-2 (1 to 2%) (633, 991). (47). Uses tryptophan or indole (750). Deficient in anthranilate phosphoribosyl transferase (1126) (Fig. 11). Scorable by blue fluorescence (anthranilate) in medium under long-wave UV after 2 to 5 days of growth on minimal medium plus indole (10 µg /ml), 34°C. Initial stocks of the first trp-4 mutant were inhibited by suboptimal concentrations of tryptophan (750), but derivatives have been obtained that are free of this problem (909).	IVR	B
lys(122)	All lysine auxotrophs are inhibited competitively by arginine (287, 288). Resistance to arginine is conferred on lys-1 mutants by a presumed transport mutation argR, q.v. See the arg entry for medium that provides both lysine and arginine requirements. Lysine biosynthesis is by the alpha-aminoadipate pathway in Neurospora and other higher fungi (see reference 1103) (Fig. 16). Complex interactions between lys, pyr, and argmutations have been described (485). Enzymes of lysine biosynthesis are derepressed coordinately with those of arginine, histidine, and tryptophan (1131). See cpc-1.Biosynthetic pathway of lysine, showing sites of gene action of lys-4and lys-5and probable sites of lys-2 and lys-3 gene action (119, 400, 464, 762, 1087). alpha-amino-epsilon-hydroxycaproic acid can be converted to alpha-aminoadipate semialdehyde (1178), but apparently is not an intermediate.		B
tolC			B