FGSC #7344

Reporting Genes: al-2 nic-1 het-i

Species: crassa

Allele: 15300 3416 no#

Alternate Strain Number: 9204

Depositor: DDP

Linkage Group: IR IR I or II

Mating Type: a

Species Number: 10

ref1: Pittenger and Brawner 1961. Genetics 46:1645-1663, https://doi.org/10.1093/genetics/46.12.1645

Genes

Locus	Cultural Requirements	Link Group	Type
nic-1	IR. Right of ace-3 (<1%), lys-1 (1%), and In(OY323). Left of os-1(10 to 29%) (2, 57, 131, 578, 789, 816, 907). (482) Uses nicotinic acid or nicotinamide, but not precursors (97, 100) (Fig. 18). Accumulates quinolinic acid (100). Used to study intralocus recombination (907). Called the q locus.	IR	B
al-2	IR. Right of os-5 (<1%) and T(STL76). Left of arg-6(1%) and al-1 (797, 802, 808, 816, 818). Included in duplications from Tp(T54M94), confirming location left of arg-6(808). (482) Carotenoids absent or abnormal, but steroids produced (398). Blocked in microsomal fraction and defective in phytoene synthetase (445), a particulate enzyme (445 and references cited therein) (Fig. 9). Tracer experiments indicate a lesion between prephytoene pyrophosphate and phytoene (572). Alleles include those resulting in white and pale rose-white, e.g., 15300 and Y254MI65 (1042), and purple, e.g., MN58a (154). For complementation, see references 500 and 1041. Fine-structure mapping (500, 1042) needs reevaluation because of new information on the location of the arg-6 marker (797).	IR	B
het-I	I or II. Linked to T(IR;IIR)4637 al-1(831).Recognized by cessation of growth of forced heterokaryons under certain conditions. het-Inuclei eliminate het-i if initial frequency exceeds 30% het-I. When more than 80% are het-i, growth of a forced heterokaryon can continue without a change of ratio. Called I and i. (831)	I or II	B

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