FGSC #7309

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Reporting Genes: (mo(R2374) A + a[m1] ad-3B cyh-1)

Species: crassa

Allele: R2374(3828-3) + helper

Alternate Strain Number: 3351

Depositor: DDP

Linkage Group: IIIR

Mating Type: A

Species Number: 10

Opposite Mating Type: 7310

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
ad-3B	IR. Between ad-3A (0.1 to 0.7%) and nic-2 (3%) (271). (482) Uses adenine or hypoxanthine (682). Blocked in interconversion of AIR to CAIR (348) (Fig. 8). Produces purple pigment, permitting direct visual selection (276, 682). Pigment is secreted with low concentrations of adenine (e.g., 0.1 mM), not with high concentrations (2 mM) (276, 682, 785). Pigment production used to assess effect of histidine and tryptophan on purine nucleotide synthesis (786). Reduced interallelic fertility (264, 407). Complementation maps (268, 274). Relation of mutagens to complementation patterns (269). Mutants with non-polarized complementation patterns on the right side of the complementation map grow on minimal medium if supplied with CO2; other mutants do not respond to CO2, (270). Used extensively for mutagenesis (see ad-3A). Rearrangement T(I- >III)Y112M4i ad-3B, which has a breakpoint inseparable from ad-3B, was the first insertional translocation to be reported for fungi (266). Allele 7-017-0137 shows "fixed instability," mutating to an unstable prototrophic allele (41). Alleles 2-17-126, 12-21-28, and numerous others are supersuppressible (408, 749, 955). Called complementation group B.	IR	B
am1			B
cyh-1	IR. Right of nit-1 (6%). Left of T(STL76) and al-2 (8 to 13%) (496, 797, 808).Resistant to cycloheximide (496, 748). Resistance is recessive in duplications (1090). Dominance reported in forced heterokaryons (496, 748) may have been due to skewed nuclear ratios (1090). Protein synthesis on ribosomes of the mutant cyh-1 proceeds in the presence of cycloheximide in a cell-free system (834). Readily scored on slants with 10 µg of cycloheximide per ml autoclaved in the medium. Excellent as a marker and valuable for selecting somatic recombinants or deletions in heterozygous duplications (748, 1091). Used to show that the cycloheximide-induced phase shift of the circadian clock involves protein synthesis (738). Called act-1: actidione resistant-1.	IR	B
A			B
mo(R2374)	Name and symbol used by Garnjobst and Tatum (382) for a miscellaneous group of mutants having spreading growth on agar, sometimes with scanty or fine hyphae and reduced conidiation. The symbol morph has also been used. Other categories of morphological mutants were designated col, spco, or smco. Other workers have assigned descriptive names, e.g., bal, fr, ro, and sc. See also moe. For reviews covering morphological mutants and morphogenesis, see references 112, 197, 642, 675, 942, 946, and 1088. Growth rates and hyphal diameters of 18 morphological mutants are given in reference 197.		B