FGSC #7242

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Reporting Genes: al-2 R un-18

Species: crassa

Allele: 15300 35408 T54M94

Alternate Strain Number: 1597

Depositor: DDP

Linkage Group: IR IR IR

Mating Type: a

Species Number: 10

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
al-2	IR. Right of os-5 (<1%) and T(STL76). Left of arg-6(1%) and al-1 (797, 802, 808, 816, 818). Included in duplications from Tp(T54M94), confirming location left of arg-6(808). (482) Carotenoids absent or abnormal, but steroids produced (398). Blocked in microsomal fraction and defective in phytoene synthetase (445), a particulate enzyme (445 and references cited therein) (Fig. 9). Tracer experiments indicate a lesion between prephytoene pyrophosphate and phytoene (572). Alleles include those resulting in white and pale rose-white, e.g., 15300 and Y254MI65 (1042), and purple, e.g., MN58a (154). For complementation, see references 500 and 1041. Fine-structure mapping (500, 1042) needs reevaluation because of new information on the location of the arg-6 marker (797).	IR	B
R	IR. Right of T(NM169d) and aro-8 (4%). Left of un-18(11%) (1093). (H.R. Cameron, personal communication) All eight ascospores of heterozygous R/+ asci are round rather than ellipsoid. R is thus nonautonomous in ascospores and dominant in the ascus (690). Ascospores are round even in nonlinear asci (1008; D.D. Perkins, unpublished data). Usually two germination pores are formed, but sometimes one (1008). Photograph (1008). Vegetative morphology abnormal, somewhat resembling that of pe mutants. Initial growth on slants is concentrated around the inoculation point. The vegetative morphology is recessive in heterozygous duplications, as from T(NM103) (1091). Female sterile, with no perithecia, but R x R crosses can be accomplished if R is heterokaryotic in the female parent (A.M. Srb, personal communication). Used in studies of duplication instability (1091) and autonomy of ascospore development (529). Allelic recurrences obtained (1008; A.M. Srb, personal communication). For other genetically determined round ascospores, see reference 59. Called Rsp (1008), but the original symbol R has priority. (rsp has been used for cytoplasmically determined respiration-deficient mutants [890].)	IR	B
un-18	IR. Right of T(NM169d) and R (11%) (808). Unknown function. Heat sensitive (507). No growth at 34°C. Growth at 25 C is substantial but not wild type, and better on complete medium than on minimal medium. Allele T54M94 is called 41 in reference 507.	IR	B