FGSC #7231

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Reporting Genes: al-1 lys-3

Species: crassa

Allele: JH612 4545

Alternate Strain Number: 1719

Depositor: DDP

Linkage Group: IR IR

Mating Type: A

Species Number: 10

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
al-1	IR. Right of hom (<1%), arg-6 (<1 to 4%), T(T54M94), and al-2. Left of lys-3 (9%). (797, 808; D.D. Perkins, unpublished data). (482) Carotenoids abnormal. Strains carrying the various alleles differ widely in phenotype, ranging from white (e.g., 4637) and "aurescent" (pigment in peripheral conidia and conidiophores, 34508) to yellow mycelia and conidia (e.g., ALS4 and RES-25). See, for example, reference 1042. Strains carrying alleles ALS-14, RES-6, 34508, and RES-25 contain large amounts of phytoene (99 to 100% of the total neutral carotenoids), suggesting a lesion that affects phytoene dehydrogenase (398, 1039) (see Fig. 9). Strains carrying allele RWT-ylo accumulate zeta carotene and smaller amounts of neurosporene, suggesting a leaky block of the step between these intermediates (1071). It is not known whether phytoene dehydrogenase catalyzes the whole series of dehydrogenations or whether leakiness of this enzyme accounts for the different mutant phenotypes. For complementation tests, see references 500, 1039, and 1041. Fine-structure mapping (500, 1042). Translocation T(4637), inseparable from al-1, was the first albino mutation and one of the first chromosome rearrangements in Neurospora to be identified and studied (656). Allele 34508 called aur: aurescent.	IR	B
lys-3	IR. Right of al-1 (9%) and al-2 (12 to 15%). Left of In(OY323) and nic-1 (<1%) (2, 907). Not included in duplications from In(OY323) x In(NM176); hence, left of ace-3(57). (288)Uses lysine or epsilon-hydroxynorleucine. Probably blocked in conversion of alpha-aminoadipate to alpha-aminoadipate semialdehyde, based on precursor utilization (400, 1087) (Fig. 16). Complementation between alleles (13). Ascospores are white and inviable in homozygous lys-3 x lys-3 crosses, but some heteroallelic crosses are fertile (13). Inhibited by methionine. Initial allele: 4545.	IR	B