FGSC #7228

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Reporting Genes: ta acr-3

Species: crassa

Allele: D317 KH14

Alternate Strain Number: 1701

Depositor: DDP

Linkage Group: IL IL

Mating Type: A

Species Number: 10

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
acr-3	IL. Between un-16 (1 to 5%) and suc (1 to 5%). Probably right of ta (816; PB). (498)Resistant to acriflavine and to malachite green (three alleles tested). Not resistant to 3-aminotriazole. Resistance is probably dominant (heterokaryon tests) (498). Scoring is clear-cut with uniform inocula of appropriate size. False-negative or false-positive scoring may result if test inocula are too small or too large. May show delayed resistance: read tests at 2 and 4 days, 34°C. Use acriflavine at 10 µg /ml in minimal agar at 34°C (816) and malachite green at 2 µg /ml (498).	IL	B
ta	IL. Between un-16 (2%) and acr-3 (< 3%) (816, PB). Rapid-spreading colonies. Morphology is distinguishable from that of the wild type, but varies with growth conditions (816). Conidiation best at 34°C. Possible maternal effect, with ta+ sometimes resembling ta in initial cultures from ascospores. (D. Newmeyer, unpublished data).	IL	B