FGSC #7227

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Reporting Genes: cyt-1 leu-4

Species: crassa

Allele: C115 R359

Alternate Strain Number: 1796

Depositor: DDP

Linkage Group: IL IL

Mating Type: a

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group
cyt-1	IL. Between leu-3 (5 to 8%) (583) and T(OY321) (D.D. Perkins, N.B. Raju, and E.G. Barry, in preparation). (694) Deficient in cytochromes aa3 and b. Very slow growth. Female sterile (87, 694). Scoring aided by slower growth on complete medium relative to that on minimal, presumably owing to inhibition by yeast extract (694, 816). cyt-U-9 is linked and may be allelic with the original cyt-1 mutation C115 (87; H. Bertrand, personal communication).	IL
leu-4	IL. Right of T(OY321); hence, of cyt-1. Left of cys-5(<<1%) (1125; D.D. Perkins, N.B. Raju, and E.G. Barry, in preparation). (429)Requires leucine, Structural gene for alpha-isopropylmalate synthetase (426, 427, 432) (Fig. 15). Feedback-negative mutants (426, 427). Hybrid synthetases with altered properties (432). Complementation between alleles (432, 1125).	IL