FGSC #7213

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Reporting Genes: ser-6 ad-8

Species: crassa

Allele: DK42 Y226M58

Alternate Strain Number: 6143

Depositor: DDP

Linkage Group: VIL VIL

Mating Type: a

Species Number: 10

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
ser-6	VIL. Between nit-6 (10%) and ad-8 (16%) (PB). (544)Responds to serine. Slight response to glycine. Extremely leaky. Scorable by slow, sparse conidiation on minimal slants or auxoanographically (D.D. Perkins, unpublished data). Can be crossed on minimal crossing medium without supplement. Allele DK42 obtained as putative leucine regulatory mutation (544), but a regulatory role is dubious (S.R. Gross, personal communication). Called DK42 (544). Probably allelic with T(VL;VIL)OY325 ser, which has a breakpoint left of lys-5(11%) in VIL, and shows 0/28 recombination with DK42, which it resembles in leakiness. The mutant OY325 grows more profusely than the mutant DK42 on minimal slants (PB).	VIL	B
ad-8	VIL. Right of ser-6 (15%) and het-8 (12%). Left of aro-6 (8%) and cpl-1(6 to 11%) (437, 510, 730, PB). Requires adenine; cannot use hypoxanthine (526). Lacks adenylosuccinate synthase (511) (Fig. 8). Fine-structure mapping and intralocus complementation (510-512). Has little hypoxanthine uptake and little hypoxanthine phosphoribosyltransferase; both these effects are partly counteracted in ad-1 ad-8 double mutants (903). Little hypoxanthine phosphoribosyltransferase is also found in mep(3) and mep(10)mutants, q.v. Used to study purine transport (787, 903, and references therein). Called complementation group E.	VIL	B