FGSC #7212

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Reporting Genes: chol-2 nit-6

Species: crassa

Allele: 47904 OP4

Alternate Strain Number: 6138

Depositor: DDP

Linkage Group: VIL VIL

Mating Type: A

Species Number: 10

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
chol-2	VIL. Left of nit-6 (6 to 8%) (812, PB). Requires choline (471). Also uses di- but not monomethylaminoethanol (468) (Fig. 12). Deficient in S-adenosylmethionine:phosphatidyl- monomethylethanolamine methyltransferase (222, 923, 924). Strains carrying the only allele, 47904t, are leaky on minimal medium at 22°C but not at 34°C (501). Phospholipid composition is abnormal on limiting choline (501). Growth is colonial on limiting supplement at 34°C and on minimal medium at 25°C.	VIL	B
nit-6	VIL. Right of chol-2 (6%). Left of ser-7 (10%) and ad-8(17%) (PB; O.C. Yoder, personal communication). Unable to use nitrate or nitrite as a nitrogen source (185). Lacks nitrite reductase (185) (Fig. 19), which is subject to positive nitrogen metabolite repression (186). Affected by nit-2 and MS5 regulator genes (838, 1076a) (see nmr-1). Used to study repression of nitrate reductase (26) and nonenzymatic reduction of nitrite (185). Induced by nitrite (198).	VIL	B