FGSC #7178

Reporting Genes: acr-3 leu-1 his-7

Species: crassa

Allele: KH5(r) 33757 Y152M31

Alternate Strain Number: 3152

Depositor: DDP

Linkage Group: IIIC IIIR IIIR

Mating Type: a

Species Number: 10

ref1: Turner & Perkins 1979 Genetics 93:587-606, https://doi.org/10.1093/genetics/93.3.587

Genes

Locus	Cultural Requirements	Link Group	Type
acr-3	IL. Between un-16 (1 to 5%) and suc (1 to 5%). Probably right of ta (816; PB). (498)Resistant to acriflavine and to malachite green (three alleles tested). Not resistant to 3-aminotriazole. Resistance is probably dominant (heterokaryon tests) (498). Scoring is clear-cut with uniform inocula of appropriate size. False-negative or false-positive scoring may result if test inocula are too small or too large. May show delayed resistance: read tests at 2 and 4 days, 34°C. Use acriflavine at 10 µg /ml in minimal agar at 34°C (816) and malachite green at 2 µg /ml (498).	IL	B
his-7	IIIR. Between leu-1 (8 to 20%) and thi-2 (1 to 2%) (2199 1052, PB). (162, 1123)Requires histidine. Blocked before imidazole glycerol phosphate (162, 1123) (Fig. 14). Intralocus recombination (172).	IIIR	B
leu-1	IIIR. Between ad-4 (1 to 5%) and his-7 (8%) (219, 578, 815, 1052). (868)Requires leucine (867, 868). Lacks beta-isopropylmalate dehydrogenase (426) (Fig. 15). Accumulates alpha-isopropylmalate and beta-isopropylmalate (427). Synthesis of the enzyme also requires the function of regulatory gene leu-3+ and the presence of alpha-isopropylmalate, which acts as inducer (427). Resistant to aminotriazole (D. D. Perkins, unpublished data). Female sterile (O. M. Mylyk, personal communication). Used to study reversion and competition in heterokaryons (901).	IIIR	B

Neurospora Crassa Wikipedia