FGSC #7162

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Reporting Genes: leu-1 thi-2

Species: crassa

Allele: 33757 9185

Alternate Strain Number: 3328

Depositor: DDP

Mating Type: a

Species Number: 10

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
thi-2	IIIR. Between his-7 (1 to 2%) and ad-2 (1 to 3%) (219, 814). (504). Requires thiamine. Cannot use pyrilmidine plus thiazole (1059). Does not undergo growth adaptation on minimal medium (302).	IIIR	B
leu-1	IIIR. Between ad-4 (1 to 5%) and his-7 (8%) (219, 578, 815, 1052). (868)Requires leucine (867, 868). Lacks beta-isopropylmalate dehydrogenase (426) (Fig. 15). Accumulates alpha-isopropylmalate and beta-isopropylmalate (427). Synthesis of the enzyme also requires the function of regulatory gene leu-3+ and the presence of alpha-isopropylmalate, which acts as inducer (427). Resistant to aminotriazole (D. D. Perkins, unpublished data). Female sterile (O. M. Mylyk, personal communication). Used to study reversion and competition in heterokaryons (901).	IIIR	B