FGSC #7147

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Reporting Genes: cot-5 het-C pyr-4 thr-2

Species: crassa

Allele: R2479 C 36601 35423

Alternate Strain Number: 2261

Depositor: DDP

Linkage Group: IIL L L L

Mating Type: A

Species Number: 10

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
cot-5	IIL. Right of T(P2869) and T(B18). Probably left of pyr-4 (0/39). Linked to fs-1 (29%) (PB). (382) Little or no growth at 34°C; colonial at 30°C. Morphology still not normal at 25°C, a temperature at which older colonies form short aerial hyphae (382). Female sterile. Morphology distinct from that of the mutant fs-1 (PB).	IIL	B
het-c	IIL. Left of pyr-4 (1%). Not included in duplications from T(AR18) or T(P2869); hence, right of cys-3 and het-6 (729, 808, PB). (378)Stable heterokaryons are not formed by strains that are het-C + het-c (378, 379); strains carrying het-C/het-c duplications show inhibited "brown flat" morphology, spreading to cover the slant but not conidiating (794, 803). Putative multiple alleles, suggested by abnormal duplication phenotypes when chromosomes from various natural sources were tested, may be due instead to additional het loci in the segment tested (729, 795). Photograph: see Fig. 3 of reference 729.	IIL	B
pyr-4	IIL. Right of het-c (1%), T(P2869), and cys-3 (18 to 21%). Left of ro-3 (1 to 2%) (721, 816, PB). (812). Requires uracil or other pyrimidine. Lacks orotidine 5'-monophosphate decarboxylase (133, 134, 841) (Fig. 20). Fertile crosses homozygous for pyr-4 can be made by using very high levels of uridine (15 to 20 mg/ml) (O.M. Mylyk, personal communication).	IIL	B
thr-2	IIL. Right of T(NM149) and ro-3 (6 to 25%). Left of T(AR179); hence, left of bal and arg-5 (3 to 18%). Adjoins thr-3 (< 0.1%) (808, 812, PB). Requires threonine. Cannot use other amino acids (1061). Lacks threonine synthetase (352) (Fig. 17). Strongly inhibited by methionine (320, 1061). Known alleles are not heat sensitive, unlike all known thr-3 alleles.	IIL	B