FGSC #7053

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Reporting Genes: fl;wc-1

Species: crassa

Allele: P;P829

Alternate Strain Number: 9271

Depositor: DDP

Linkage Group: IIR;VIIR

Mating Type: a

Species Number: 10

Genetic Background: SL

Opposite Mating Type: 7052

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
fl	IIR. Between ace-1 (5 to 11%) and trp-3 (3%) (816, PB). (613)No macroconidia (609). Highly fertile (612). Used routinely as the female parent in tests for chromosome rearrangements and for mating type (e.g., reference 801). The flsingle mutant produces few microconidia when dry; when wetted, sufficient microconidia are produced to have been used in early irradiation and mutation studies (614, 915); large numbers can be obtained under certain conditions; see reference 893. pe fl (46, 700) and fl;dn (806) double mutants produce abundant microconidia; the latter combination is highly fertile when homozygous. Photograph of microconidial formation (774); see also reference 893. Nuclear numbers in microconidia (46, 64, 478). Wall analysis (207). Immunoelectrophoretic pattern (784). Paradoxical high alcoholic glycolysis on nitrate medium (80). Deficiency of isocitrate lyase on acetate medium; see citations in reference 1088. When fl A and fl a strains are inoculated separately on crossing medium in plates, a double line of perithecia forms where they meet, similar to that accompanying barrage in Podospora (410, 414). fl ascospores from certain fl x fl+ crosses often germinate spontaneously (1127; N. B. Raju, personal communication). Allele C-1835 was called acon (717, 812).	IIR	B
wc-1	VIIR. Right of met-9 (1 to 4%). Left of un-10 (7%) and for (6%) (724, 812, 816). Carotenoids absent from mycelia; conidia become pigmented with some delay. Named because nonconidiating rim at top of agar slant remains white. A double mutant with flor other nonconidiating mutant would be classed as albino. Regulatory mutants for photoinduced carotenogenesis via blue light receptor might be expected to have a similar phenotype (444, 445). A blue light treatment (given in vivo), which increases the activity of soluble and microsomal enzymes required for phytoene biosynthesis in the wild type, does not do so in the mutant wc-1 (445). Fails to show phototropism of perithecial beaks when used as the female (protoperithecial) parent, but not when used as the male (fertilizing) parent (R. W. Harding, personal communication). Useful genetic marker (725, 800). Scoring clearest at high temperatures (34 C).	VIIR	B