FGSC #7025

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Reporting Genes: leu-4 leu-1 ipm-1^+ ipm-2^+

Species: crassa

Allele: R59 D221

Alternate Strain Number: R59,F84-2-61a

Depositor: SRG

Mating Type: a

Species Number: 10

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
leu-1	IIIR. Between ad-4 (1 to 5%) and his-7 (8%) (219, 578, 815, 1052). (868)Requires leucine (867, 868). Lacks beta-isopropylmalate dehydrogenase (426) (Fig. 15). Accumulates alpha-isopropylmalate and beta-isopropylmalate (427). Synthesis of the enzyme also requires the function of regulatory gene leu-3+ and the presence of alpha-isopropylmalate, which acts as inducer (427). Resistant to aminotriazole (D. D. Perkins, unpublished data). Female sterile (O. M. Mylyk, personal communication). Used to study reversion and competition in heterokaryons (901).	IIIR	B
leu-4	IL. Right of T(OY321); hence, of cyt-1. Left of cys-5(<<1%) (1125; D.D. Perkins, N.B. Raju, and E.G. Barry, in preparation). (429)Requires leucine, Structural gene for alpha-isopropylmalate synthetase (426, 427, 432) (Fig. 15). Feedback-negative mutants (426, 427). Hybrid synthetases with altered properties (432). Complementation between alleles (432, 1125).	IL	B
ipm-2	Unmapped. Unlinked to ipm-1 or leu-4.Improves alpha-isopropylmalate uptake by the mutant ipm-1 in supporting growth of the mutant leu-4(870, 871). By itself ipm-2 is not very effective in promoting permeability of alpha-isopropylmalate.		B
ipm-1	Unmapped. Unlinked to ipm-2 or leu-4.Able to use alpha-isopropylmalate to support growth of leu-4 mutants and for induction of alpha-isopropylmalate isomerase and beta-isopropylmalate dehydrogenase, in contrast to ipm+ strains, which are unable to take up this intermediate (870, 871).		B