FGSC #7008

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Reporting Genes: leu-1;leu-2

Species: crassa

Allele: D221;R86

Alternate Strain Number: R86-20-39a

Mutagen: UV

Depositor: SRG

Linkage Group: IIIR;IVR

Mating Type: a

Species Number: 10

Opposite Mating Type: 7007

ref1: Gross 1962. PNAS 48:922-930, https://doi.org/10.1073/pnas.48.6.922

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
leu-1	IIIR. Between ad-4 (1 to 5%) and his-7 (8%) (219, 578, 815, 1052). (868)Requires leucine (867, 868). Lacks beta-isopropylmalate dehydrogenase (426) (Fig. 15). Accumulates alpha-isopropylmalate and beta-isopropylmalate (427). Synthesis of the enzyme also requires the function of regulatory gene leu-3+ and the presence of alpha-isopropylmalate, which acts as inducer (427). Resistant to aminotriazole (D. D. Perkins, unpublished data). Female sterile (O. M. Mylyk, personal communication). Used to study reversion and competition in heterokaryons (901).	IIIR	B
leu-2	IVR. Between trp-4 (2%) and ilv-3 (4%) (579, 991). (D.C. Regnery, cited in reference 633)Requires leucine (867, 868). Structural gene for isopropylmalate isomerase (432, 871) (Fig. 15). Altered heat inactivation of hybrid enzymes (432). Structural differences of hybrid enzymes (871). Accumulates alpha-isopropylmalate (427). Synthesis of the enzyme also requires the function of regulatory gene leu-3+ and the presence of alpha-isopropylmalate, which acts as an inducer (427). Resistant to aminotriazole (D.D. Perkins, unpublished data). Alleles show intralocus complementation (424). Allele 37501 is heat sensitive (30°C versus 20 C) and is leaky at 25°C (D.C. Regnery, personal communication).	IVR	B