FGSC #6997

Reporting Genes: leu-2

Species: crassa

Allele: D176

Alternate Strain Number: D176a

Mutagen: UV

Depositor: SRG

Linkage Group: IVR

Mating Type: a

Species Number: 10

ref1: Gross 1962. PNAS 48:922-930, https://doi.org/10.1073/pnas.48.6.922

Genes

Locus	Cultural Requirements	Link Group	Type
leu-2	IVR. Between trp-4 (2%) and ilv-3 (4%) (579, 991). (D.C. Regnery, cited in reference 633)Requires leucine (867, 868). Structural gene for isopropylmalate isomerase (432, 871) (Fig. 15). Altered heat inactivation of hybrid enzymes (432). Structural differences of hybrid enzymes (871). Accumulates alpha-isopropylmalate (427). Synthesis of the enzyme also requires the function of regulatory gene leu-3+ and the presence of alpha-isopropylmalate, which acts as an inducer (427). Resistant to aminotriazole (D.D. Perkins, unpublished data). Alleles show intralocus complementation (424). Allele 37501 is heat sensitive (30°C versus 20 C) and is leaky at 25°C (D.C. Regnery, personal communication).	IVR	B

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