FGSC #6931

Reporting Genes: Sk-3 acr-7 (Sk from N. intermedia)

Species: crassa

Allele: -- P1676(r)

Alternate Strain Number: 3220

Depositor: BCT

Linkage Group: IIIL L

Mating Type: A

Species Number: 10

Opposite Mating Type: 6932

ref1: Campbell and Turner 1987. Genome 29:129, https://doi.org/10.1139/g87-022

ref2: https://doi.org/10.1128/mr.46.4.426-570.1982

Genes

Locus	Cultural Requirements	Link Group	Type
acr-7	IIIL. Left of sc (12 to 14%). Linked to thi-4 (7%) (PB). Right of r(Sk-2)-1 (7%) (B.C. Turner, personal communication). Report of VI linkage in reference 818, is incorrect.Resistant to acriflavine (50 µg /ml). Not cross-resistant to 3-aminotriazole or malachite green. Several acr-7 strains have become female infertile after vegetative transfer. (PB)	IIIL	B
Sk-3	Near the centromere. The killer allele Sk-3K suppresses crossing over in the interval r(Sk-2)-1-leu-1 (29% in controls). Recombines with his-7 (11%) but not with leu-1 (0/72) or acr-7 (0/60,000) and only 3/19,000 with acr-2. (1092; B.C. Turner, personal communication) Origin and characteristics resemble those of Sk-2, q.v. However, Sk-3K strains are sensitive to killing by Sk-2K and vice versa. The killer allele was found in N. intermedia, introgressed into N. crassa. Most wild-type strains of both species are sensitive, but resistant strains of N. intermedia have been found. Strains resistant to Sk-3K are not necessarily resistant to Sk-2K (857, 1092).		B

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