FGSC #6878

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Reporting Genes: (nd al-2 nic-1 + pan-2)

Species: crassa

Allele: no# 15300 S1413 + Y153M66

Alternate Strain Number: ndH

Depositor: BSR

Linkage Group: IR R R + VIR

Mating Type: a

Species Number: 10

Genetic Background: SL

ref1: Seidel-Rogol et al 1989. Mol Cell Biol 9:4259, https://doi.org/10.1128/mcb.9.10.4259-4264.1989

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
al-2	IR. Right of os-5 (<1%) and T(STL76). Left of arg-6(1%) and al-1 (797, 802, 808, 816, 818). Included in duplications from Tp(T54M94), confirming location left of arg-6(808). (482) Carotenoids absent or abnormal, but steroids produced (398). Blocked in microsomal fraction and defective in phytoene synthetase (445), a particulate enzyme (445 and references cited therein) (Fig. 9). Tracer experiments indicate a lesion between prephytoene pyrophosphate and phytoene (572). Alleles include those resulting in white and pale rose-white, e.g., 15300 and Y254MI65 (1042), and purple, e.g., MN58a (154). For complementation, see references 500 and 1041. Fine-structure mapping (500, 1042) needs reevaluation because of new information on the location of the arg-6 marker (797).	IR	B
nic-1	IR. Right of ace-3 (<1%), lys-1 (1%), and In(OY323). Left of os-1(10 to 29%) (2, 57, 131, 578, 789, 816, 907). (482) Uses nicotinic acid or nicotinamide, but not precursors (97, 100) (Fig. 18). Accumulates quinolinic acid (100). Used to study intralocus recombination (907). Called the q locus.	IR	B
pan-2	VIR. Right of rib-1 (<1 to 3%). Left of del (6%) and trp-2(11%) (140, 141, 143, 818, PB). Unable to convert ketovaline to ketopantoic acid (138, 140, 141). Used in major studies of intralocus recombination and complementation (140-143). pan-2ascospores remain white or pale if the crossing medium is not supplemented, even when the protoperithecial parent is pan-2+. Asci in which gene conversion has occurred at pan-2 can thus be recognized and isolated (1072, 1073); photographs (1072). For good recovery of pan-2progeny, crossing media should be supplemented with pantothenic acid (10 µg/ml) even when the protoperithecial parent is pan+. Called group B.	VIR	B
nd	IR. Between the centromere (15%) and al-2 (20%) (981).Decreasing clonal growth potential under all nutritional conditions, followed by abrupt irreversible cessation of growth (707, 981). Hypersensitive to sorbose. Conidia die rapidly on slants at 4°C (707). Recessive in heterokaryons. An aged strain can be rejuvenated through heterokaryosis or by crossing to nd+. Extracts nontoxic (981). Used to examine hypotheses of senescence based on faulty protein synthesis (607) and lipid autoxidation with free-radical reactions (702). Stocks maintained in balanced heterokaryons. Initial growth rate of the original strain, 2.5 mm/h; however, nd progeny free of modifiers grow initially at 4.5 mm/h (wild-type rate) (707).	IR	B