FGSC #6868

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Reporting Genes: pi (=col-10)

Species: crassa

Allele: R2438

Depositor: FGSC

Linkage Group: IIL

Mating Type: a

Species Number: 10

Genetic Background: SL

Opposite Mating Type: 6845

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
pi	IIL. Linked to ro-7 (0/75); left of cys-3 (4%) (816). Spreading growth (allele B101); no conidia. (816) Growth is better on minimal medium than on complex complete medium. The mutant pi (B101) has not been tested for 6-phosphogluconate dehydrogenase, which is deficient in strain(s) carrying putative allele col-10 (R2438) (947). See col-10regarding allelism. As a marker, pi (B101) is preferable to col-10 (R2438) because of growth rate, stability, and ease of handling. ro-7, in the same region, is preferable to both (PB).	IIL	B
col-10	IIL. Linked to cys-3 (14%), near or at pi. One wild type in 81 progeny from a col-10 x pi cross may have been a revertant (382, 816).Slow growing; dense, compact morphology without conidia (382). Altered 6-phosphogluconate dehydrogenase (947, 949). (col-3 also affects 6-phosphogluconate dehydrogenase.) col-10 (R2438) x pi (B101) crosses resemble R2438 x R2438 and B101 x B101 crosses in producing abnormal asci with flaccid walls and unordered ascospores, suggesting allelism (1007). However, R2438 and B101 mutants are distinctly different in morphology. Mutant B101 has not been tested for 6-phosphogluconate dehydrogenase. As a marker, pi (B101) is preferable to R2438 because of growth rate, stability, and ease of handling; ro-7 in the same region is preferable to both (PB).	IIL	B