FGSC #6820

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Reporting Genes: al-2;mus-16;pan-2

Species: crassa

Allele: Y112M38;JMB15.2;Y387-15.7a

Alternate Strain Number: C2-T100-14A

Mutagen: M

Depositor: HI

Linkage Group: IR;VL;VIR

Mating Type: A

Species Number: 10

Genetic Background: SL3

Opposite Mating Type: 6821

ref1: Inoue and Schroeder Mut. Res. 194:9-16 1988, https://doi.org/10.1016/0167-8817(88)90051-x

ref2: Baker et al Mut. Res. 132:171-179 1984, https://doi.org/10.1016/0167-8817(84)90035-x

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
al-2	IR. Right of os-5 (<1%) and T(STL76). Left of arg-6(1%) and al-1 (797, 802, 808, 816, 818). Included in duplications from Tp(T54M94), confirming location left of arg-6(808). (482) Carotenoids absent or abnormal, but steroids produced (398). Blocked in microsomal fraction and defective in phytoene synthetase (445), a particulate enzyme (445 and references cited therein) (Fig. 9). Tracer experiments indicate a lesion between prephytoene pyrophosphate and phytoene (572). Alleles include those resulting in white and pale rose-white, e.g., 15300 and Y254MI65 (1042), and purple, e.g., MN58a (154). For complementation, see references 500 and 1041. Fine-structure mapping (500, 1042) needs reevaluation because of new information on the location of the arg-6 marker (797).	IR	B
mus-16	VL. Between caf-1(4%) and lys-1 (9%). Isolated as a mutant sensitive to nitrogen mustard. Defective in DNA-DNA and DNA-protein corss-link repair. Sensitive to MMS, NG, histidine, hydroxyurea, not to UV or mitomycin C	VL	B
pan-2	VIR. Right of rib-1 (<1 to 3%). Left of del (6%) and trp-2(11%) (140, 141, 143, 818, PB). Unable to convert ketovaline to ketopantoic acid (138, 140, 141). Used in major studies of intralocus recombination and complementation (140-143). pan-2ascospores remain white or pale if the crossing medium is not supplemented, even when the protoperithecial parent is pan-2+. Asci in which gene conversion has occurred at pan-2 can thus be recognized and isolated (1072, 1073); photographs (1072). For good recovery of pan-2progeny, crossing media should be supplemented with pantothenic acid (10 µg/ml) even when the protoperithecial parent is pan+. Called group B.	VIR	B