FGSC #6762

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Reporting Genes: al-1 age-1 aro-8

Species: crassa

Allele: 34508 1.5 DH8

Depositor: KDM

Linkage Group: IR R R

Mating Type: A

Species Number: 10

Genetic Background: SL

ref1: Munkres & Furtek Mech. Age. Dev. 1984 25:63-77, https://doi.org/10.1016/0047-6374(84)90130-1

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
age-1	IR. Symbol used for a series of many linked loci distal to nit-1, possibly redundant complexes. Individual loci symbolized as 1.3, 1.5, etc. Prototype age 1.5 is right of so(14%) and left of aro-8 (7.6%); age 1.3 maps at same site as so, q.v. (K.D. Munkres, personal communication). Reduced conidial longevity in the light. Not expressed in the dark, or in the light with vitamin E or reduced glutathione. Deficient in an isozyme of catalase, in mitochondrial superoxide dismutase, and in other enzymes involved in destroying free radicals and peroxides. Scored by plating efficiency after incubation of mature slant cultures at 30 C, 100% relative humidity, in continuous white fluorescent light, 24 J/square meter. Also scored by a defect in conidiophorogenesis on Vogel sorbose- sucrose plates. Initial mutants selected as spontaneous variants from f1 of Oak Ridge wild types; variants with increased conidial longevity can also be selected. High spontaneous mutation rate. Longer life span correlated with slower growth. (702, 704, 705, 708; K. D. Munkres, personal communication)	IR	B
al-1	IR. Right of hom (<1%), arg-6 (<1 to 4%), T(T54M94), and al-2. Left of lys-3 (9%). (797, 808; D.D. Perkins, unpublished data). (482) Carotenoids abnormal. Strains carrying the various alleles differ widely in phenotype, ranging from white (e.g., 4637) and "aurescent" (pigment in peripheral conidia and conidiophores, 34508) to yellow mycelia and conidia (e.g., ALS4 and RES-25). See, for example, reference 1042. Strains carrying alleles ALS-14, RES-6, 34508, and RES-25 contain large amounts of phytoene (99 to 100% of the total neutral carotenoids), suggesting a lesion that affects phytoene dehydrogenase (398, 1039) (see Fig. 9). Strains carrying allele RWT-ylo accumulate zeta carotene and smaller amounts of neurosporene, suggesting a leaky block of the step between these intermediates (1071). It is not known whether phytoene dehydrogenase catalyzes the whole series of dehydrogenations or whether leakiness of this enzyme accounts for the different mutant phenotypes. For complementation tests, see references 500, 1039, and 1041. Fine-structure mapping (500, 1042). Translocation T(4637), inseparable from al-1, was the first albino mutation and one of the first chromosome rearrangements in Neurospora to be identified and studied (656). Allele 34508 called aur: aurescent.	IR	B
aro-8	Grows on minimal medium unless both phenylalanine and tyrosine are present.	IR	B