FGSC #6443

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Reporting Genes: mus-29 ylo-1 pan-2

Species: crassa

Allele: FK119 Y30539y Y153M96

Alternate Strain Number: M2468

Depositor: EK

Linkage Group: VIL L R

Mating Type: a

Species Number: 10

Opposite Mating Type: 6442

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
ylo-1	VIL. Between cys-1 (8%) and ad-1 (6%). Probably right of Bml (2%) (1012, PB). (381). Yellow carotenoids (381). Affects synthesis of neurosporaxanthin (4'-apo-beta'-caroten-4'-oic acid); citations in reference 398. Lesion probably involves the conversion of lycopene to 3,4-dehydrolycopene or the conversion of either torulene or gamma-carotene to neurosporaxanthin (398 and references therein) (Fig. 9). Resembles the orange wild type in young cultures, but color differences become clear with age. Expressed in both conidia and mycelia. Undefined modifiers affect intensity. Fails to complement with many of the al-1 and al-2 albino strains (R.E. Subden, personal communication).	VIL	B
mus-29	VIL. Between chol-2 (14%) and lys-5 (15%). Moderately sensitive to MMS and histidine, low but consistant sensitivity to gamma rays and bleomycin, and not sensitive to UV or mitomycin C. Reduced conidial viability. Homozygous sterile. One mutant, FK119.	VIL	B
pan-2	VIR. Right of rib-1 (<1 to 3%). Left of del (6%) and trp-2(11%) (140, 141, 143, 818, PB). Unable to convert ketovaline to ketopantoic acid (138, 140, 141). Used in major studies of intralocus recombination and complementation (140-143). pan-2ascospores remain white or pale if the crossing medium is not supplemented, even when the protoperithecial parent is pan-2+. Asci in which gene conversion has occurred at pan-2 can thus be recognized and isolated (1072, 1073); photographs (1072). For good recovery of pan-2progeny, crossing media should be supplemented with pantothenic acid (10 µg/ml) even when the protoperithecial parent is pan+. Called group B.	VIR	B