FGSC #6433

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Reporting Genes: mus-27 nuc-2

Species: crassa

Allele: FK124 T28M2

Alternate Strain Number: M2358

Depositor: EK

Mating Type: a

Species Number: 10

Opposite Mating Type: 6432

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
mus-27	IIR. Right of nuc-2 (16%). Highly sensitive to gamma rays (but less so than uvs-6), MMS, bleomycin (more so than uvs-6), and histidine, sensitive to UV only at very high doses, and insensitive to mitomycin C. Homozygous fertile. One mutant, FK124.	IIR	B
nuc-2	IIR. Between the T(NM177) breakpoints; hence, right of aro-3. Left of preg(1 to 2%) and pe (4%). Probably allelic with pcon (0/854) (593, 671). (514) Unable to use RNA or DNA as a phosphorus source (514). Defective in production of repressible alkaline and acid phosphatases (671, 1077). Several nucleases absent or reduced (449). Interaction with other phosphate regulatory genes (665). Recessive to nuc+ in partial diploids and heterokaryons (671). Not defective in nuh function (538). Scored on low-phosphate medium by a staining reaction with alpha-naphthyl phosphate plus diazo blue B (397, 1077), by failure to grow on minimal medium altered so that 0.1 g of RNA or DNA per liter is substituted for the inorganic phosphate source (514, 538), or by failure to grow on low-phosphate medium at a pH above 7 (R.L. Metzenberg, personal communication). Used to study phosphate transport (624). For a regulation model, see references 665 and 670. See pcon.	IIR	B