FGSC #6125

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Reporting Genes: his-3 ad-6

Species: crassa

Allele: Y306M176

Alternate Strain Number: Y306M176

Mutagen: UV

Depositor: MEC

Linkage Group: IR

Mating Type: A

Species Number: 10

Genetic Background: SL

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
ad-6	IVR. Right of ilv-3 (9%) (579). Left of pan-1 (1 to 2%), chol-1 (1%), and cot-1 (2 to 6%) (633). (692) Uses adenine or hypoxanthine (682). Blocked in conversion of phosphoribosylformylglycineamide to phosphoribosylformylglycineamidine (120) (Fig. 8). Inhibited by caffeine in the presence of adenine (1172). Called complementation group 1.	IVR	B
his-3	IR. Right of met-10 (R.L. Metzenberg, personal communication). Left of cog (1 to 3%) (172,174), ure-4 (1%) (78), and ad-3A (1%) (271). (434)Requires histidine (434). Complex gene coding for histidinol dehydrogenase, phosphoribosyladenosine 5'-triphosphate-pyrophosphohydrolase, and phosphoribosyl-adenosine 5'-monophosphate-cyclohydrolase (16, 673) (Fig. 14). All three activities appear to be catalyzed by a single protein (673). Strains carrying different individual alleles may lack only the early reaction(s) or only histidinol dehydrogenase, or both. Those that lack only histidinol dehydrogenase accumulate histidinol (16, 162, 1123). Mutants produce cross-reacting material (220). Used to study intralocus complementation and recombination (15, 16, 27, 162, 164, 171, 172, 1121, 1122, 1124). Intralocus recombination is regulated by cog and by rec-2 (27, 171); it is not affected by rec-1 (172). Translocation T(IR;VII)TM429, with one breakpoint in his-3, has been used to show that cog is cis-acting (171). Initial alleles: C140 and T1710 (= C1710).	IR	B