FGSC #6043

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Reporting Genes: (os-4a + a[m1] ad-3B cyh-1)

Species: crassa

Allele: Y256M223 + m1 2-17-114 KH52(r)

Alternate Strain Number: 1805

Depositor: DDP

Linkage Group: I

Mating Type: a

Species Number: 10

Opposite Mating Type: 6042

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
a			B
ad-3B	IR. Between ad-3A (0.1 to 0.7%) and nic-2 (3%) (271). (482) Uses adenine or hypoxanthine (682). Blocked in interconversion of AIR to CAIR (348) (Fig. 8). Produces purple pigment, permitting direct visual selection (276, 682). Pigment is secreted with low concentrations of adenine (e.g., 0.1 mM), not with high concentrations (2 mM) (276, 682, 785). Pigment production used to assess effect of histidine and tryptophan on purine nucleotide synthesis (786). Reduced interallelic fertility (264, 407). Complementation maps (268, 274). Relation of mutagens to complementation patterns (269). Mutants with non-polarized complementation patterns on the right side of the complementation map grow on minimal medium if supplied with CO2; other mutants do not respond to CO2, (270). Used extensively for mutagenesis (see ad-3A). Rearrangement T(I- >III)Y112M4i ad-3B, which has a breakpoint inseparable from ad-3B, was the first insertional translocation to be reported for fungi (266). Allele 7-017-0137 shows "fixed instability," mutating to an unstable prototrophic allele (41). Alleles 2-17-126, 12-21-28, and numerous others are supersuppressible (408, 749, 955). Called complementation group B.	IR	B
am1			B
cyh-1	IR. Right of nit-1 (6%). Left of T(STL76) and al-2 (8 to 13%) (496, 797, 808).Resistant to cycloheximide (496, 748). Resistance is recessive in duplications (1090). Dominance reported in forced heterokaryons (496, 748) may have been due to skewed nuclear ratios (1090). Protein synthesis on ribosomes of the mutant cyh-1 proceeds in the presence of cycloheximide in a cell-free system (834). Readily scored on slants with 10 µg of cycloheximide per ml autoclaved in the medium. Excellent as a marker and valuable for selecting somatic recombinants or deletions in heterozygous duplications (748, 1091). Used to show that the cycloheximide-induced phase shift of the circadian clock involves protein synthesis (738). Called act-1: actidione resistant-1.	IR	B
os-4	I. Right of T(39311) and arg-1 (1%). Left of T(AR173)and T(AR190); hence, of un-2 and his-2. Linked to sn (0/33). (Data for allele Y256M233.) (802, PB) Sensitive to high osmotic pressure. Readily scored by morphology on nonmoist slants. Allele Y256M223, originally called flm-2, is preferred over NM201o, on which the locus designation was initially based. (802)	I	B