FGSC #5918

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Reporting Genes: caf-1 lys-1 cyt-9 at

Species: crassa

Allele: KH101 33933 17 NM221

Alternate Strain Number: 5314

Depositor: DDP

Linkage Group: VL VL VL VC

Mating Type: A

Species Number: 10

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
caf-1	VL. Right of T(OY321) (11%), T(AR30) (19%), and. hence, of NO. Left of T(AR33)(5 to 12%) and lys-1 (4 to 14%). (817, PB; K.S. Hsu, unpublished data) Resistant to caffeine (494). Resistance is dominant in duplications from T(AR33) (817). Scoring is clear at 25°C and poor at 34°C on slants with 2 or 2.5 mg of caffeine per ml of minimal medium without sorbose (R.L. Metzenberg, personal communication). Also readily scorable by using conidial suspensions spotted on plates containing 2 mg of caffeine per ml of minimal sorbose medium (PB).	VL	B
cyt-9	V. Between lys-1 (5%) and at (5%) (87, PB). Deficient in cytochromes aa3 and b. Slow growth. (87)	V	B
lys-1	V. Right of caf-1 (4 to 14%). Left of cyt-9 (5%) and at (1 to 20%) (817; K.S. Hsu, personal communication; PB). (403)Uses lysine, alpha-aminoadipic acid, or epsilon-hydroxynorleucine (alpha-amino-epsilon-hydroxycaproic acid) (399, 400, 684, 1087). Accumulates homocitrate on limiting lysine concentrations (464) (Fig. 16). Fine structure and complementation between alleles (8). Initial allele: 33933.	V	B
at	Grows on minimal media. Conidia form in clumps, especially at the top of a slant.	V	B