FGSC #5536

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Reporting Genes: ad-3B;pe fl

Species: crassa

Allele: 2-17-51;Y8743m L

Alternate Strain Number: 74-OR82-1a

Depositor: FJD

Linkage Group: IR;IIR R

Mating Type: a

Species Number: 10

Genetic Background: SL

Opposite Mating Type: 5537

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
ad-3B	IR. Between ad-3A (0.1 to 0.7%) and nic-2 (3%) (271). (482) Uses adenine or hypoxanthine (682). Blocked in interconversion of AIR to CAIR (348) (Fig. 8). Produces purple pigment, permitting direct visual selection (276, 682). Pigment is secreted with low concentrations of adenine (e.g., 0.1 mM), not with high concentrations (2 mM) (276, 682, 785). Pigment production used to assess effect of histidine and tryptophan on purine nucleotide synthesis (786). Reduced interallelic fertility (264, 407). Complementation maps (268, 274). Relation of mutagens to complementation patterns (269). Mutants with non-polarized complementation patterns on the right side of the complementation map grow on minimal medium if supplied with CO2; other mutants do not respond to CO2, (270). Used extensively for mutagenesis (see ad-3A). Rearrangement T(I- >III)Y112M4i ad-3B, which has a breakpoint inseparable from ad-3B, was the first insertional translocation to be reported for fungi (266). Allele 7-017-0137 shows "fixed instability," mutating to an unstable prototrophic allele (41). Alleles 2-17-126, 12-21-28, and numerous others are supersuppressible (408, 749, 955). Called complementation group B.	IR	B
fl	IIR. Between ace-1 (5 to 11%) and trp-3 (3%) (816, PB). (613)No macroconidia (609). Highly fertile (612). Used routinely as the female parent in tests for chromosome rearrangements and for mating type (e.g., reference 801). The flsingle mutant produces few microconidia when dry; when wetted, sufficient microconidia are produced to have been used in early irradiation and mutation studies (614, 915); large numbers can be obtained under certain conditions; see reference 893. pe fl (46, 700) and fl;dn (806) double mutants produce abundant microconidia; the latter combination is highly fertile when homozygous. Photograph of microconidial formation (774); see also reference 893. Nuclear numbers in microconidia (46, 64, 478). Wall analysis (207). Immunoelectrophoretic pattern (784). Paradoxical high alcoholic glycolysis on nitrate medium (80). Deficiency of isocitrate lyase on acetate medium; see citations in reference 1088. When fl A and fl a strains are inoculated separately on crossing medium in plates, a double line of perithecia forms where they meet, similar to that accompanying barrage in Podospora (410, 414). fl ascospores from certain fl x fl+ crosses often germinate spontaneously (1127; N. B. Raju, personal communication). Allele C-1835 was called acon (717, 812).	IIR	B
pe	IIR. Between nuc-2 (4%) and arg-12 (1 to 5%) (593, 816). (613) Peach-colored conidia and short hyphae formed, more uniformly than by the wild type, as a lawn close to surface of agar. Distinctive morphology (46, 613). Added arginine increases macroconidiation and tends to obscure scoring of peat 25 C, but not at 39°C. pe single mutants produce both macro- and microconidia. pe fl double mutants produce abundant grey microconidia and no macroconidia (46, 700) (see fl). See col-1, col-4, and references 415 and 416 for interactions with other genes. Called m (microconidial) or pem in some contexts.	IIR	B