FGSC #5412

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Reporting Genes: cel

Species: crassa

Allele: EB2853(t)

Alternate Strain Number: B2893-15a

Mutagen: S

Depositor: EGB

Linkage Group: IVR

Mating Type: a

Species Number: 10

Genetic Background: M

Opposite Mating Type: 5411

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
cel	IVR. Linked to pan-1 (1%) and cot-1 (0/17) (812). Requires saturated fatty acids. Defect attributed to impaired chain elongation (455). Deficient in the fatty acid synthetase complex (317). increased sensitivity to oligomycin (L.R. Forman and S. Brody, cited in reference 293), Tween 20 provides a convenient supplement. Requirement is "leaky" at 21 to 22°C (317) but not at 34°C (812). Used to change fatty acid composition (113) and make the circadian clock sensitive to fatty acids and temperature (114, 649). Temperature compensation of the clock is lacking in celmutants (650). Used to study membrane lipid-phase transitions and electrical properties (363, 364). Used to incorporate photolabile azido fatty acid probes for membrane studies (176). Called ol (oleic acid) (812) and fas (fatty acid synthesis) (317). Initial report of oleic acid utilization (812) was incorrect (455), probably because of impurities.	IVR	B