FGSC #5185

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Reporting Genes: chol-2 lys-5 ylo-1 pan-2

Species: crassa

Allele: 47904(t) DS6-85 Y30539y Y153M96

Alternate Strain Number: M2087

Depositor: EK

Linkage Group: VIL VIL VIR VIR

Mating Type: a

Species Number: 10

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
chol-2	VIL. Left of nit-6 (6 to 8%) (812, PB). Requires choline (471). Also uses di- but not monomethylaminoethanol (468) (Fig. 12). Deficient in S-adenosylmethionine:phosphatidyl- monomethylethanolamine methyltransferase (222, 923, 924). Strains carrying the only allele, 47904t, are leaky on minimal medium at 22°C but not at 34°C (501). Phospholipid composition is abnormal on limiting choline (501). Growth is colonial on limiting supplement at 34°C and on minimal medium at 25°C.	VIL	B
lys-5	VIL. Right of cyt-2 (6%), aro-6 (3%), and cpl-1 (5 to 7%). Left of un-4 (2%) (1012, PB).Requires lysine. Partial response to glutaric acid (762). Lacks homocitrate synthase activity (464, 762) (Fig. 16). Some alleles such as 37402, called asco, are autonomous ascospore lethals or semilethals, resulting in mostly immature white spores (see reference 1012 for a photograph). Viability of the lys- spores is improved by long incubation (7). With other alleles such as DS6-85, ascospores blacken and germinate normally. Accumulates malate plus citrate on media with limiting lysine concentrations (464). Four complementation groups (12). Allele 37402 called asco.	VIL	B
pan-2	VIR. Right of rib-1 (<1 to 3%). Left of del (6%) and trp-2(11%) (140, 141, 143, 818, PB). Unable to convert ketovaline to ketopantoic acid (138, 140, 141). Used in major studies of intralocus recombination and complementation (140-143). pan-2ascospores remain white or pale if the crossing medium is not supplemented, even when the protoperithecial parent is pan-2+. Asci in which gene conversion has occurred at pan-2 can thus be recognized and isolated (1072, 1073); photographs (1072). For good recovery of pan-2progeny, crossing media should be supplemented with pantothenic acid (10 µg/ml) even when the protoperithecial parent is pan+. Called group B.	VIR	B
ylo-1	VIL. Between cys-1 (8%) and ad-1 (6%). Probably right of Bml (2%) (1012, PB). (381). Yellow carotenoids (381). Affects synthesis of neurosporaxanthin (4'-apo-beta'-caroten-4'-oic acid); citations in reference 398. Lesion probably involves the conversion of lycopene to 3,4-dehydrolycopene or the conversion of either torulene or gamma-carotene to neurosporaxanthin (398 and references therein) (Fig. 9). Resembles the orange wild type in young cultures, but color differences become clear with age. Expressed in both conidia and mycelia. Undefined modifiers affect intensity. Fails to complement with many of the al-1 and al-2 albino strains (R.E. Subden, personal communication).	VIL	B