FGSC #5180

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Reporting Genes: chol-2 ylo-1 trp-2

Species: crassa

Allele: 47904(t) Y30539y 41

Alternate Strain Number: M2033

Depositor: EK

Linkage Group: VIL VIL VIR

Mating Type: A

Species Number: 10

Opposite Mating Type: 5181

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
chol-2	VIL. Left of nit-6 (6 to 8%) (812, PB). Requires choline (471). Also uses di- but not monomethylaminoethanol (468) (Fig. 12). Deficient in S-adenosylmethionine:phosphatidyl- monomethylethanolamine methyltransferase (222, 923, 924). Strains carrying the only allele, 47904t, are leaky on minimal medium at 22°C but not at 34°C (501). Phospholipid composition is abnormal on limiting choline (501). Growth is colonial on limiting supplement at 34°C and on minimal medium at 25°C.	VIL	B
ylo-1	VIL. Between cys-1 (8%) and ad-1 (6%). Probably right of Bml (2%) (1012, PB). (381). Yellow carotenoids (381). Affects synthesis of neurosporaxanthin (4'-apo-beta'-caroten-4'-oic acid); citations in reference 398. Lesion probably involves the conversion of lycopene to 3,4-dehydrolycopene or the conversion of either torulene or gamma-carotene to neurosporaxanthin (398 and references therein) (Fig. 9). Resembles the orange wild type in young cultures, but color differences become clear with age. Expressed in both conidia and mycelia. Undefined modifiers affect intensity. Fails to complement with many of the al-1 and al-2 albino strains (R.E. Subden, personal communication).	VIL	B
trp-2	VIR. Right of del (0 to 13%). Left of un-23 (5 to 27%), T(OY320), and ws-1 (38%) (818, 822, 1019, PB). Uses kynurenine, anthranilic acid, indole, or tryptophan (96). Kynurenine is utilized by conversion to anthranilate (447). Inferred to be the structural gene for the alpha subunit of the anthranilate synthetase complex (546). The gene product catalyzes anthranilate synthesis with ammonia but not with glutamine as the amino donor (29). Specifies anthranitate synthetase (glutamine linked) in collaboration with trp-1 in trifunctional trp-1+-trp-2+ enzyme aggregate (181, 259) (Fig. 11); see trp-1. Nonsense allele used to isolate supersuppressors (954) and to study enzyme complex restored by supersuppressors (183).	VIR	B