FGSC #5095

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Reporting Genes: (ser-3 un-16 acr-3 a + a[m1] ad-3B cyh-1)

Species: crassa

Allele: 47903 T42M69 KH14(r) + m1 B114 KH52(r)

Alternate Strain Number: 1787 (10003

Depositor: DDP

Linkage Group: IL

Mating Type: a

Species Number: 10

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
ser-3	IL. Right of cys-5 (<1%). Left of un-3 (<1%) and In(NM176) (8I6, 1093, PB). Uses serine and grows less well on formate. Also grows fairly well on a combination of adenine, methionine, tryptophan, and lysine. Does not grow on casein hydrolysate (290). No or little response to glycine (D.D. Perkins, unpublished data). Deficient in phosphoserine phosphatase activity (70). Inhibited by leucine. Scorability good, but vigor and leakiness vary markedly in different isolates. Homozygous crosses give mostly white ascospores (816; D.D. Perkins, unpublished data). Alleles, 47903 and JBM5.	IL	B
a			B
cyh-1	IR. Right of nit-1 (6%). Left of T(STL76) and al-2 (8 to 13%) (496, 797, 808).Resistant to cycloheximide (496, 748). Resistance is recessive in duplications (1090). Dominance reported in forced heterokaryons (496, 748) may have been due to skewed nuclear ratios (1090). Protein synthesis on ribosomes of the mutant cyh-1 proceeds in the presence of cycloheximide in a cell-free system (834). Readily scored on slants with 10 µg of cycloheximide per ml autoclaved in the medium. Excellent as a marker and valuable for selecting somatic recombinants or deletions in heterozygous duplications (748, 1091). Used to show that the cycloheximide-induced phase shift of the circadian clock involves protein synthesis (738). Called act-1: actidione resistant-1.	IR	B
am1			B
ad-3B	IR. Between ad-3A (0.1 to 0.7%) and nic-2 (3%) (271). (482) Uses adenine or hypoxanthine (682). Blocked in interconversion of AIR to CAIR (348) (Fig. 8). Produces purple pigment, permitting direct visual selection (276, 682). Pigment is secreted with low concentrations of adenine (e.g., 0.1 mM), not with high concentrations (2 mM) (276, 682, 785). Pigment production used to assess effect of histidine and tryptophan on purine nucleotide synthesis (786). Reduced interallelic fertility (264, 407). Complementation maps (268, 274). Relation of mutagens to complementation patterns (269). Mutants with non-polarized complementation patterns on the right side of the complementation map grow on minimal medium if supplied with CO2; other mutants do not respond to CO2, (270). Used extensively for mutagenesis (see ad-3A). Rearrangement T(I- >III)Y112M4i ad-3B, which has a breakpoint inseparable from ad-3B, was the first insertional translocation to be reported for fungi (266). Allele 7-017-0137 shows "fixed instability," mutating to an unstable prototrophic allele (41). Alleles 2-17-126, 12-21-28, and numerous others are supersuppressible (408, 749, 955). Called complementation group B.	IR	B
acr-3	IL. Between un-16 (1 to 5%) and suc (1 to 5%). Probably right of ta (816; PB). (498)Resistant to acriflavine and to malachite green (three alleles tested). Not resistant to 3-aminotriazole. Resistance is probably dominant (heterokaryon tests) (498). Scoring is clear-cut with uniform inocula of appropriate size. False-negative or false-positive scoring may result if test inocula are too small or too large. May show delayed resistance: read tests at 2 and 4 days, 34°C. Use acriflavine at 10 µg /ml in minimal agar at 34°C (816) and malachite green at 2 µg /ml (498).	IL	B
un-16	IL. Right of mt (<1%). Left of ta (1%) and acr-3(<3%). Closest flanking marker right of the mating type locus (818, PB). Unknown function. Heat sensitive, 34°C versus 25°C (507). Allele T42M69 is called 16 in reference 507.	IL	B