FGSC #5093

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Reporting Genes: al-2 hom

Species: crassa

Allele: 15300 51504

Alternate Strain Number: 1146

Depositor: DDP

Linkage Group: IR

Mating Type: A

Species Number: 10

Opposite Mating Type: 5094

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
hom	IR. Right of arg-6 (1%), Tp(T54M94), and al-2 (2 to 7%). Between the breakpoints of T(STL76) and T(4637); hence, left of al-1 (cnr (1%), probably to the left (789, 812). (1061, 1063)Uses homoserine, or methionine plus threonine (1063). Affects aspartate beta-semialdehyde dehydrogenase (518) (see Fig. 17). Inhibited on complex complete medium and by methionine and other amino acids (1063); supplemented minimal medium should therefore be used. Symbol changed from hs.	IR	B
al-2	IR. Right of os-5 (<1%) and T(STL76). Left of arg-6(1%) and al-1 (797, 802, 808, 816, 818). Included in duplications from Tp(T54M94), confirming location left of arg-6(808). (482) Carotenoids absent or abnormal, but steroids produced (398). Blocked in microsomal fraction and defective in phytoene synthetase (445), a particulate enzyme (445 and references cited therein) (Fig. 9). Tracer experiments indicate a lesion between prephytoene pyrophosphate and phytoene (572). Alleles include those resulting in white and pale rose-white, e.g., 15300 and Y254MI65 (1042), and purple, e.g., MN58a (154). For complementation, see references 500 and 1041. Fine-structure mapping (500, 1042) needs reevaluation because of new information on the location of the arg-6 marker (797).	IR	B