FGSC #5089

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Reporting Genes: cr lys-4 al-2

Species: crassa

Allele: LP957

Depositor: DDP

Linkage Group: IR

Mating Type: A

Species Number: 10

Genetic Background: OR

ref1: 1973 LePage Heredity 34:293 (Abstr)

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
cr			B
lys-4	IR. Between nuc-1 (1%) and his-3 (1%) (271, 670). (288)Requires lysine (400). Lacks saccharopine-cleaving enzyme activity (1087) (Fig. 16). Cornplementation between alleles (13). Use 0.5 mg of lysine per ml. Initial allele: 15069.	IR	B
al-2	IR. Right of os-5 (<1%) and T(STL76). Left of arg-6(1%) and al-1 (797, 802, 808, 816, 818). Included in duplications from Tp(T54M94), confirming location left of arg-6(808). (482) Carotenoids absent or abnormal, but steroids produced (398). Blocked in microsomal fraction and defective in phytoene synthetase (445), a particulate enzyme (445 and references cited therein) (Fig. 9). Tracer experiments indicate a lesion between prephytoene pyrophosphate and phytoene (572). Alleles include those resulting in white and pale rose-white, e.g., 15300 and Y254MI65 (1042), and purple, e.g., MN58a (154). For complementation, see references 500 and 1041. Fine-structure mapping (500, 1042) needs reevaluation because of new information on the location of the arg-6 marker (797).	IR	B