FGSC #5079

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Reporting Genes: ser-3 un-16 acr-3

Species: crassa

Allele: 47903 T42M69 KH14

Alternate Strain Number: 10003-2

Depositor: DDP

Linkage Group: IL L L

Mating Type: a

Species Number: 10

Genetic Background: OR

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
un-16	IL. Right of mt (<1%). Left of ta (1%) and acr-3(<3%). Closest flanking marker right of the mating type locus (818, PB). Unknown function. Heat sensitive, 34°C versus 25°C (507). Allele T42M69 is called 16 in reference 507.	IL	B
acr-3	IL. Between un-16 (1 to 5%) and suc (1 to 5%). Probably right of ta (816; PB). (498)Resistant to acriflavine and to malachite green (three alleles tested). Not resistant to 3-aminotriazole. Resistance is probably dominant (heterokaryon tests) (498). Scoring is clear-cut with uniform inocula of appropriate size. False-negative or false-positive scoring may result if test inocula are too small or too large. May show delayed resistance: read tests at 2 and 4 days, 34°C. Use acriflavine at 10 µg /ml in minimal agar at 34°C (816) and malachite green at 2 µg /ml (498).	IL	B
ser-3	IL. Right of cys-5 (<1%). Left of un-3 (<1%) and In(NM176) (8I6, 1093, PB). Uses serine and grows less well on formate. Also grows fairly well on a combination of adenine, methionine, tryptophan, and lysine. Does not grow on casein hydrolysate (290). No or little response to glycine (D.D. Perkins, unpublished data). Deficient in phosphoserine phosphatase activity (70). Inhibited by leucine. Scorability good, but vigor and leakiness vary markedly in different isolates. Homozygous crosses give mostly white ascospores (816; D.D. Perkins, unpublished data). Alleles, 47903 and JBM5.	IL	B