FGSC #4964

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Reporting Genes: arg-1 rg-1 cr-1 thi-1 al-2

Species: crassa

Allele: 46004 B53 B123 56501 15300

Alternate Strain Number: 46004-16-22

Depositor: ELT

Linkage Group: IL IR IR IR IR

Mating Type: a

Species Number: 10

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
al-2	IR. Right of os-5 (<1%) and T(STL76). Left of arg-6(1%) and al-1 (797, 802, 808, 816, 818). Included in duplications from Tp(T54M94), confirming location left of arg-6(808). (482) Carotenoids absent or abnormal, but steroids produced (398). Blocked in microsomal fraction and defective in phytoene synthetase (445), a particulate enzyme (445 and references cited therein) (Fig. 9). Tracer experiments indicate a lesion between prephytoene pyrophosphate and phytoene (572). Alleles include those resulting in white and pale rose-white, e.g., 15300 and Y254MI65 (1042), and purple, e.g., MN58a (154). For complementation, see references 500 and 1041. Fine-structure mapping (500, 1042) needs reevaluation because of new information on the location of the arg-6 marker (797).	IR	B
arg-1	Uses arginine but not precursors.	IL	B
cr-1	IR. Right of ace-7 (1 to 3%) and nic-2 (4 to 7%). Left of cys-9 (3%) and un-1(5%) (721, 816). Included in duplications from T(4540), which do not include cr-2 or cr-3(PB). (610) Rapid conidiation close to surface of agar. Produces very short conidiophores, bearing conidia in tight clusters (610, 611). Photographs (533, 634). Recessive. Deficient in adenylate cyclase (1066); has little or no endogenous adenosine 3',5'-phosphate (1065, 779). Abnormal morphology partially corrected by exogenous adenosine 3',5'-phosphate (891, 892, 1065, 1066). Guanosine 3',5'-phosphate also stimulates mycelial elongation (892). Cyclic nucteotide levels differ in mycelia and conidia (891, 892). NAD(P) glycohydrolase is overproduced and excreted; this is normalized by adenosine 3',5'-phosphate (533). Induction and localization of p-glucosidase is altered; induction is normalized by adenosine 3',5'-phosphate (906). Inability to use glycerol and certain other carbon sources is also overcome by adenosine 3',5'phosphate (598, 1067). Phosphodiesterase inhibitors do not counteract the morphological effect of cr-1 (892). Increased lactate dehydrogenase activity (92). Used to determine what functions are controlled by adenosine 3',5'-phosphate (779). Used to study adenosine 3',5'-phosphate binding protein (1082). Strains carrying the various alleles vary in growth habit (B123 strains are flat, restricted; allele L strains are spreading, but morphology may vary on different media). Modifier mutations which alter morphology and the ability of cr-1 to use glycerol occur frequently (383, 905). Crosses homozygous for allele B123 exude intact linear asci (634). Double mutants sn cr and cr rg form small conidiating colonies suitable for replica plating with velvet (182, 634, 796, 932, 1020). The triple mutant sn cr;csp-2 can be overlayered (744; photograph 747). The single mutant (B123) can be replicated by using a needle replicator (634). Scorability and viability are good. Excellent as a marker. Carotenoids formed normally. cr-1 ascospores may require longer to mature than cr+ ascospores. Allele CE4-11-67 called con(716, 717).	IR	B
rg-1	IR. Right of T(AR173); hence, of his-2. Left of lys-4 (1 to 7%) (271, 789, 810).Spreading dense colonial growth with poor conidiation (789). Increased hyphal branching; bumpy mycelial surface. Altered phosphoglucomutase (isozyme I). Accumulates glucose-1-phosphate (117). Cell wall composition (132). Normal levels of NADPH (110) and linolenic acid (115). Photograph (112). The double mutant rg cr grows as small discrete conidiating colonies suitable for velvet replication (634). For examples of applications, see references 932 and 1020. Unlike sn cr, which it resembles phenotypically, the double mutant rg cr is not homozygous fertile. Allele R2357 was formerly called er: erupt (see reference 382). Allele S4357 was formerly called col-7 (see reference 675).	IR	B
thi-1	IR. Right of the T(4540) right breakpoint and cys-9 (13%). Left of T(NM103), T(ALS182), and met-6 (7 to 14%) (721, 808, 816, 1091). (482). Uses thiamine or precursors pyrimidine plus thiazole (1059). Adaptation to growth on minimal medium occurs after a lag; growth tests should, therefore, be scored early. Adaptation is not carried over via ascospores, conidia, or small mycelial fragments. Adaptive growth is paralleled by attainment of wild-type thiamine pyrophosphate and carboxylase levels. Apparently concerns utilization of intact thiamine rather than its biosynthesis. (302, 303). Allele 17084 is inseparable from translocation T(IR;VII)17084 (808).	IR	B