FGSC #4962

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Reporting Genes: Not available; pdx-1 col-4 ad-6 pan-1 rug

Species: crassa

Allele: 37803 B148 28610 5531 B57

Alternate Strain Number: 37-20A

Depositor: BDM

Linkage Group: IVR IVR IVR IVR IVR

Mating Type: A

Species Number: 10

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
ad-6	IVR. Right of ilv-3 (9%) (579). Left of pan-1 (1 to 2%), chol-1 (1%), and cot-1 (2 to 6%) (633). (692) Uses adenine or hypoxanthine (682). Blocked in conversion of phosphoribosylformylglycineamide to phosphoribosylformylglycineamidine (120) (Fig. 8). Inhibited by caffeine in the presence of adenine (1172). Called complementation group 1.	IVR	B
col-4	IVR. Between met-1 (4%) and arg-2 (<1 to 2%) (692, 876, 991). (695) Spreading colonial morphology, forming dense balls of conidia high in slants (47). Probably dominant in heterozygous duplications from T(S1229) (E.G. Barry, personal communication). Cell wall-autolyzing enzyme (631). Reduced amount of cell wall peptides (1165). Used in combination with pe fl to produce microconidiating colonial growth suitable for reversion experiments (386). Called spco-1 (382); called c (386).	IVR	B
rug	IVR. Right of pyr-2 (3%). Left of the T(NMI52) right breakpoint and cys-4 (10%) (633, 721,812).Spreading colonial morphology, with conidiating tufts. Grows better on sucrose minimal medium than on glycerol complete medium (789).	IVR	B
pan-1	IVR. Between ad-6 (1 to 2%) and cot-1 (2 to 3%) (633, 692, PB). (482). cel, col-1, int, pho-3, and thi-5 all appear to be closely linked in this crowded region. Requires intact pantothenic acid for growth under standard conditions. Able to synthesize both precursors, beta-alanine and pantoyl lactone (1058). Ability to synthesize pantothenic acid from beta-alanine plus pantoyl lactone is demonstrable in vitro but not in vivo unless cultures are aerated (1111, 1113, 1114). Unlike pan-2, pan-1 has no effect on ascospore ripening in heterozygous crosses. Called group A. For alleles see reference 138.	IVR	B
pdx-1	IVR. Right of pyr-1 (<1 to 10%). Left of T(S1229) and pt (2%) (40, 55, 692, 808). (482) Uses pyridoxine, pyridoxal, or pyridoxamine (843, 845, 846). Shows intralocus complementation (845, 846) and recombination (848), Provided the first proven example of gene conversion (686). Scoring is sharpened by addition of 100 mg of desoxypyridoxine per liter (845). Several alleles (called pdxp: e.g., 44602) are pH sensitive and can grow without pyridoxine on medium containing ammonium ions at a pH above 6 (1029). Conidia are subject to death by unbalanced growth on minimal medium (1033). A yellow pigment is excreted under certain conditions by the pdx-1;En(pdx) double mutant; see En(pdx). Allele 44204 originally called pdx-2 (see reference 848).	IVR	B