FGSC #4776

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Reporting Genes: pan-2;al-2;cot-1;uvs-6

Species: crassa

Alternate Strain Number: 74-OR270-71A

Depositor: FJD

Linkage Group: VIR;VR;IVR;IR

Mating Type: A

Species Number: 10

Opposite Mating Type: 4777

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
uvs-6	IR. Between thi-1 (3 to 8%) and ad-9 (4%) (538; E. Kafer, personal communication; D. Newmeyer, unpublished data). Very close to met-6 (< 1%) (D. Newmeyer, unpublished data). (937). Increases sensitivity to UV (273, 937), ionizing radiation (537, 937, 940), nitrosoguanidine (509, 537), histidine (755), methyl methane sulfonate (536), ICR-170 and 4-nitroquinoline 1-oxide (509), and possibly mitomycin C (537). Normal UV-induced mutation (273). For mutation induction by other agents, see references 509 and 940. Increased spontaneous mutation not evident in the ad-3 system (275), but is found for recessive lethals in a heterokaryon test system (E. Kafer, personal communication). Normal dimer excision (1164). Defective in extracellular nuclease, giving reduced halos around colonies on DNA agar (538). Increased duplication instability due to mitotic recombination, deletion, or both (759). Homozygous barren (759), with a block at crozier differentiation (860). Reduced conidial viability (537). Switches to stop-start growth after initial normal growth (D. Newmeyer, unpublished data). Not completely recessive in heterokaryotic conidia (979). Increased stability of CPS (Pyr) activity in vitro suggests that protease activity may be reduced in the mutant uvs-6 (882); see also reference 360. Double mutant uvs-3;uvs-6 is inviable (506).	IR	B
al-2	IR. Right of os-5 (<1%) and T(STL76). Left of arg-6(1%) and al-1 (797, 802, 808, 816, 818). Included in duplications from Tp(T54M94), confirming location left of arg-6(808). (482) Carotenoids absent or abnormal, but steroids produced (398). Blocked in microsomal fraction and defective in phytoene synthetase (445), a particulate enzyme (445 and references cited therein) (Fig. 9). Tracer experiments indicate a lesion between prephytoene pyrophosphate and phytoene (572). Alleles include those resulting in white and pale rose-white, e.g., 15300 and Y254MI65 (1042), and purple, e.g., MN58a (154). For complementation, see references 500 and 1041. Fine-structure mapping (500, 1042) needs reevaluation because of new information on the location of the arg-6 marker (797).	IR	B
cot-1	IVR. Between pan-1 (2%) and his-4 (1 to 6%) (692, 812, 816). Extremely colonial at 34°C, but completely normal growth, morphology, and fertility at 25°C and below. Linear growth is maximum at 24°C (374). Becomes colonial at 32°C; colonies from ascospores or conidia are viable and continue to grow slowly with dense branching, but do not conidiate. They quickly resume normal growth when shifted to a permissive temperature (692, 1068). Recessive in duplications (808); apparent dominance in heterokaryons (374) may have resulted from a shift in nuclear ratios. Used in studies of septation and branching (202), growth-inhibiting mucopolysaccharide (878, 879), and sulfate transport (641). Cell wall analysis (374). Growth is stimulated by lysine or arginine (0.1 mM) on glucose media at high temperatures (615). Because of high viability and tightly restricted growth at restrictive temperatures and normality at 25°C, cot-1 mutants have valuable technical applications. For example, crosses homozygous for cot-1 have been used in combination with sorbose for experiments with rec genes, where high-density ascospore platings are required for precise quantitative analysis of intralocus recombination (e.g., references 165, 997, and 1070). In another application, when shifted up after initial growth at the permissive low temperature, cot-1hyphae assume a "bottle brush" appearance with small side branches (692). This has been used to select uvs mutants by subsurface survival on UV-irradiated plates containing p-aminobenzoic acid (938; D.E.A. Catcheside, personal communication). cot-1 conidia or ascospores from cot-1 x cot-1crosses are used for replication in a protocol involving transfer by filter paper (615). For suppressors of cot-1, see gul.	IVR	B
pan-2	VIR. Right of rib-1 (<1 to 3%). Left of del (6%) and trp-2(11%) (140, 141, 143, 818, PB). Unable to convert ketovaline to ketopantoic acid (138, 140, 141). Used in major studies of intralocus recombination and complementation (140-143). pan-2ascospores remain white or pale if the crossing medium is not supplemented, even when the protoperithecial parent is pan-2+. Asci in which gene conversion has occurred at pan-2 can thus be recognized and isolated (1072, 1073); photographs (1072). For good recovery of pan-2progeny, crossing media should be supplemented with pantothenic acid (10 µg/ml) even when the protoperithecial parent is pan+. Called group B.	VIR	B