FGSC #4761

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Reporting Genes: fz;sg;os-1

Species: crassa

Allele: no#;no#;B135

Alternate Strain Number: fz.sg.os-1v

Depositor: GAS

Linkage Group: --;--;IR

Mating Type: A

Species Number: 10

ref1: Scarborough, G.A. 1985. Exp. Mycol. 9:275-278, https://doi.org/10.1016/0147-5975(85)90025-8

ref2: https://doi.org/10.4148/1941-4765.1681

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
fz	Unmapped.Abnormal morphology; one component of the combination of mutant genes that results in the cell-wall-less "slime" phenotype (321).		B
os-1	IR. Between nic-1 (10 to 29%) and arg-13 (1%) (789, 812, 816). (M.R. Emerson, cited in reference 789) Sensitive to high osmotic pressure. Readily scored by morphology on nonmoist slants or by failure to grow on media with 4% NaCl. Most os-1 alleles result in cultures that form no or few conidia on agar slants. Alleles NM233t and NM204t are heat sensitive (25°C versus 34°C). In media of high osmolarity, os-1 strains form protoplasts (323, 438). os-1 (Bl35) is an essential genotypic component of the wall-less strain slime (321). Protoplasts of strains carrying heatsensitive allele NM233t are stable at 37 C, with a 7.5-h redoubling time, and show good regeneration. The biochemical defect differs from that affected by either polyoxin or sorbose (chitin or glucan syntheses) (970, 971). Cell wall pores are four times larger in an os-1 mutant than in the wild type; os-1 also has a higher exclusion threshold and a 30-fold-higher galactosamine/ glucosamine ratio (1083, 1084). Intralocus complementation (676). Allele Y256M209 called flm-1.	IR	B
sg	Unmapped. Ascospores germinate without heat shock. Usually associated with a very poor vegetative growth habit. A component of the multiply mutant combination resulting in the cell-wall-less "slime" phenotype. Possibly more complex than a single gene (321). See slime.		B