FGSC #4690

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Reporting Genes: cys-3 eas

Species: crassa

Allele: NM27(t) UCLA191

Alternate Strain Number: MLS 90-39

Depositor: MLS

Linkage Group: IIL IIR

Mating Type: a

Species Number: 10

Opposite Mating Type: 4689

ref1: NN 32:12-13, https://doi.org/10.4148/1941-4765.1570

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
eas	IIR. Linked to rip-1 (1/151), trp-3 (0/71), and fl (1/52) (PB). (967)Conidia and aerial hyphae are readily wetted by water; in contrast, those of wild type are hydrophobic. Resembles csp mutants in that conidia do not readily become airborne, but differs from csp mutants in that conidia do not remain joined in the proconidial chains (967). Rodlets are lacking from the surface of conidia (75). Conidiating cultures can be scored by adding a drop of water to the culture, by tapping an inverted slant (967), or by transferring conidia to liquid (PB). Not scorable in combination with fl. Somewhat sensitive to high osmotic pressure (PB). A class of slow-growing progeny is produced from crosses heterozygous or homozygous for eas(811).	IIR	B
cys-3	IIL. Right of pi (4%). Left of T(AR18) and pyr-4 (18 to 21%) (721, 808, 816).Uses sulfite, cysteine, or methionine; little or no response to thiosulfate (640, 721). Regulator of genes of sulfur uptake and metabolism (e.g., sulfate permease, aryl sulfatase, choline sulfatase) (284, 640, 667). Grows well on methionine. Resistant to chromate (640). Used extensively for studying regulation; for a review, see reference 642. cys-3 ascospores darken slowly or not at all, even when a cys-3 strain is the fertilizing parent and when a strain carrying heat-sensitive allele NM27t is crossed at 25°C, the permissive temperature for growth. Adding methionine to crossing medium promotes darkening but fails to give good recovery of cys-3 progeny. Recovery of a few percent cys-3 progeny is possible in well-aged crosses. (721, PB). cys-3 can be used effectively as an autonomous ascospore color mutant for demonstrating segregation patterns in asci (811; see reference 858 for photograph; see Ascospore color mutants).	IIL	B