FGSC #4685

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Reporting Genes: inl;eas

Species: crassa

Allele: 37401;UCLA191

Alternate Strain Number: MLS85-5

Depositor: MLS

Linkage Group: VR;IIR

Mating Type: A

Species Number: 10

Opposite Mating Type: 4686

ref1: NN 32:12-13, https://doi.org/10.4148/1941-4765.1570

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
eas	IIR. Linked to rip-1 (1/151), trp-3 (0/71), and fl (1/52) (PB). (967)Conidia and aerial hyphae are readily wetted by water; in contrast, those of wild type are hydrophobic. Resembles csp mutants in that conidia do not readily become airborne, but differs from csp mutants in that conidia do not remain joined in the proconidial chains (967). Rodlets are lacking from the surface of conidia (75). Conidiating cultures can be scored by adding a drop of water to the culture, by tapping an inverted slant (967), or by transferring conidia to liquid (PB). Not scorable in combination with fl. Somewhat sensitive to high osmotic pressure (PB). A class of slow-growing progeny is produced from crosses heterozygous or homozygous for eas(811).	IIR	B
inl	VR. Between pho-3 (3 to 4%) and pab-1 (1 to 10%). Right of al-3 (362, 397, 1036). (482)Requires inositol (65). Lacks D-myoinositol-1-phosphatase (1142). Lack of glucocycloaldolase found by Pina and Tatum (826) is attributed by Williams (1142) to drastic repression of glucocycloaldolase by the concentration of inositol used for growth. Growth is colonial on low levels of inositol (367). Tends to extrude dark pigment into the medium when grown on suboptimal inositol. Composition of phospholipids and cell walls is abnormal on limiting inositol (367, 439, 440, 501). Inhibited by hexachlorocyclohexane (366, 457, 931). Conidia are subject to death by unbalanced growth on minimal medium (1028, 1033), a property exploited for mutant enrichment ("inositol-less death") (606, 647) because double mutants are at a selective advantage. Heat-sensitive allele 83201 is especially useful for mutant enrichment (832, 1043). Used in the first experiments reporting transformation of Neurospora by N. crassaDNA (677, 679) and reported to be efficient as a recipient in absence of inositol (1162). Used to study glucose (917) and sulfate (641) transport systems. Used extensively for studying induced reversion (392). Used for studying the mechanism of inositol-less death (647, 702), mutagenicity of ferrous ions, and regulation of mitochondrial membrane fluidity; for a review, see reference 702. Spontaneous reversion rates (386). Allele-specific partial suppressor (390). Allele 46802 is nonrevertable and inseparable from translocation 46802 (386, 808). Strains carrying heat-sensitive allele 83201 show slow semicolonial growth in liquid minimal medium at 25°C (641), but look normal on slants (D.D. Perkins, unpublished data). Strains carrying allele 89601 contain cross-reacting material (1183). Mutant gene exo-1 is present in the inl(89601) a stock FGSC 498 and may, therefore, be present in stocks of mutants derived by inositol-less death. (See references 194, 325, and 1027). Called inos.	VR	B