FGSC #4654

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Reporting Genes: cot-2;eas

Species: crassa

Allele: R1006;UCLA191

Alternate Strain Number: MLS 89-6

Depositor: MLS

Linkage Group: VR;IIR

Mating Type: a

Species Number: 10

Opposite Mating Type: 4653

ref1: NN 32:12-13, https://doi.org/10.4148/1941-4765.1570

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
eas	IIR. Linked to rip-1 (1/151), trp-3 (0/71), and fl (1/52) (PB). (967)Conidia and aerial hyphae are readily wetted by water; in contrast, those of wild type are hydrophobic. Resembles csp mutants in that conidia do not readily become airborne, but differs from csp mutants in that conidia do not remain joined in the proconidial chains (967). Rodlets are lacking from the surface of conidia (75). Conidiating cultures can be scored by adding a drop of water to the culture, by tapping an inverted slant (967), or by transferring conidia to liquid (PB). Not scorable in combination with fl. Somewhat sensitive to high osmotic pressure (PB). A class of slow-growing progeny is produced from crosses heterozygous or homozygous for eas(811).	IIR	B
cot-2	VR. Right of pk (8%) and ser-2 (5%). Left of ad-7(4%) (156, 818). (698) Recombines with inv (5%) (315).Small colonies at 34°C but fully viable. Growth and morphology nearly normal at 25°C but not completely so (382). Makes altered invertase; it is not clear whether cot-2 is the structural gene for a second subunit or whether cot-2affects structure indirectly, e.g., by altering the carbohydrate moiety (315). See inv. Reduced amount of cell wall peptides (1165). Ascospores are normal in heterozygous crosses, but are round in homozygous cot-2 x cot-2crosses (59). Some cot-2 strains carry mei-3, which was found in the original cot-2 strain (757); however, most strains used by Eggerding et al. (315) are free of it, and mei-3 cannot be responsible for the effects on invertase (D. Newmeyer, unpublished data).	VR	B