FGSC #4652

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Reporting Genes: ad-3;eas

Species: crassa

Allele: 38701;UCLA191

Alternate Strain Number: MLS 87-36

Depositor: MLS

Linkage Group: IR;IIR

Mating Type: a

Species Number: 10

Opposite Mating Type: 4651

ref1: NN 32:12-13, https://doi.org/10.4148/1941-4765.1570

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
ad-3	IR. Between his-3 (1 to 2%) and ad-3B (0.1 to 0.7%) (271). Right of ure-4 (78). (482) Requires adenine or hypoxanthine (682). Blocked in interconversion of CAIR plus aspartate to SAICAR (348) (Fig. 8). Produces purple pigment, permitting direct visual selection (276, 682); see the ad-3B entry. Reduced interallelic fertility (407). No interallelic complementation (267; F.J. de Serres, personal communication). ad-3A and ad-3B are two genetically and functionally distinct loci separated by a short but functionally complex region of unknown but essential function (271, 407). They have been used intensively for quantitative genetic and molecular studies of mutation (for a review, see reference 35). Either forward mutation (e.g., reference 277) or reverse mutation (e.g., reference 772) can be measured precisely; the former is detected visually by purple pigment. Purple pigment has also been used to assess the effect of histidine and tryptophan on purine nucleotide synthesis (786). Alleles N23 and N24 have been used as mutagen testers. N23 reverts with agents that cause base pair substitutions; N24 reverts with agents that cause frameshifts (772). SK(ad-3A) is at or near ad-3A and may be a cryptic ad-3A allele. Does not require adenine. In SK(ad-3A)x ad-3Acrosses, the ad-3A progeny die; possibly SK(ad-3A) mutants fail to make enough adenine to support their growth (251). Translocations Y155M64 ad-3A (272; PB) and Y112M15 ad-3A(413) each have one breakpoint that is inseparable from ad-3A. Called complementation group A (264). "A" in the locus symbol does not refer to mating type.	IR	B
eas	IIR. Linked to rip-1 (1/151), trp-3 (0/71), and fl (1/52) (PB). (967)Conidia and aerial hyphae are readily wetted by water; in contrast, those of wild type are hydrophobic. Resembles csp mutants in that conidia do not readily become airborne, but differs from csp mutants in that conidia do not remain joined in the proconidial chains (967). Rodlets are lacking from the surface of conidia (75). Conidiating cultures can be scored by adding a drop of water to the culture, by tapping an inverted slant (967), or by transferring conidia to liquid (PB). Not scorable in combination with fl. Somewhat sensitive to high osmotic pressure (PB). A class of slow-growing progeny is produced from crosses heterozygous or homozygous for eas(811).	IIR	B