FGSC #4650

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Reporting Genes: ro-7 arg-5 rip-1

Species: crassa

Allele: R2470 27947 4m(t)

Alternate Strain Number: 2229

Depositor: DDP

Linkage Group: IIL IIR IIR

Mating Type: a

Species Number: 10

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
ro-7	IIL. Linked to pi (0/75). Left of cys-3 (11%) (382, PB)Resembles ro-1 (382). Growth limited on glycerol medium (212). Female sterile, contrary to misprint in reference 811.	IIL	B
arg-5	Uses ornithine, citrulline or arginine.	IIR	B
rip-1	IIR. Linked to un-15 (1%); right of fl and trp-3 (6 to 9%). (PB). Conditional defect in production of 60S ribosomal subunits (622). At the restrictive temperature (37 C), RNA synthesis is affected first and then protein synthesis; 60S cytosolic ribosomal subunits are underaccumulated, and relatively little 25S rRNA is produced (618, 620, 622; P.J. Russell, personal communication). Defective ribosome biosynthesis at high temperatures is attributed to a defect in rRNA processing (622). Attains 2.4% of the wild-type growth rate at 35°C and 80% at 25°C (P.J. Russell, personal communication). Scored as an irreparable heat-sensitive un mutant (see un). Good fertility, growth, and viability make rip-1preferable to un-15 as a marker for the right end of II. The onginal strain carrying both rip-1 and inl (89601) (382, PB). was called 4M(t). The rip-1 allele in this strain was originally called 1(t).	IIR	B