FGSC #4616

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Reporting Genes: mei-3;uvs-2

Species: crassa

Allele: N289;no#

Alternate Strain Number: S-825

Depositor: ALS

Linkage Group: IL;IVR

Mating Type: A

Species Number: 10

ref1: Schroeder & Olsen 1984 Can J Gen Cytol 25:16-25, https://doi.org/10.1139/g83-003; https://doi.org/10.1139/g68-115

ref2: Newmeyer & Galenzzi 1978 Genetics 89:245-269, https://doi.org/10.1093/genetics/89.2.245

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
mei-3	IL. Right of arg-1 (3%). Probably right of eth-1 (1%) and arg-3 (1%). Left of the T(39311)right breakpoint; hence, left of the centromere and sn (757, 808).Homozygous barren (757). Recessive. Blocks meiosis in zygotene (860). Sensitive to UV, histidine (757, 759), mitomycin C (195), ionizing radiation, and methyl methane sulfonate (939). Sensitivity to UV and histidine is temperature sensitive; best scored at 39 C, at least for strains carrying allele N289 (757). Causes increased duplication instability (mitotic recombination, deletion, or both) (757).	IL	B
uvs-2	IVR. Right of cys-4 (5%) (1023). Left of pmb (8%) (S. Ogilvie-Villa, cited in reference 248; R. Sadler and S. Ogilvie-Villa, personal communication) and the T(S4342) right breakpoint. Linked to T(AR209), T(T54M50), and T(ALS179) (2 to 6%), which mark the IVR tip (808). Sensitive to UV (273, 1023), ionizing radiation (537, 935, 940), methyl methane sulfonate (536, 537), nitrosoguanidine (509, 935), mitomycin C (537), 4-nitroquinoline 1-oxide (509), nitrous acid (D.R. Stadler and E. Crane, personal communication), and ICR-170 (509). Slight or no sensitivity to histidine (537, 759). No dimer excision (1164). Normal spontaneous mutation (275). High UV-induced mutation rate (273); for mutation induction by other agents, see references 509 and 940. Homozygous fertile; no effect on meiosis or crossing over. Recessive in heterokaryons (1023). uvs-2 is the most UV sensitive of Neurosporamutants (15 to 20 times wild type) (537, 938). Used to show that DNA repair is induced by a small dose of UV (1022). Used to demonstrate postreplication repair (130). Only known allele was discovered in several Seattle stocks of mixed ancestry, and thus may be present in lab stocks elsewhere (1023; D.R. Stadler, personal communication). Not to be confused with a cytoplasmically determined UV-sensitive mutation called uvs-2 in reference 187, but now called [uvs(cyt)].	IVR	B