FGSC #4610

Reporting Genes: nmr-1

Species: crassa

Allele: MS-5

Alternate Strain Number: FS671

Depositor: RMD

Linkage Group: VR

Mating Type: a

Species Number: 10

Genetic Background: SL

Opposite Mating Type: 4609

ref1: Premakumar, Sorger & Gooden 1980 J Bact 144:542-51, https://doi.org/10.1128/jb.144.2.542-551.1980

ref2: DeBusk & Ogilvie 1984 J. Bacteriol. Nov. 1984, https://doi.org/10.1128/jb.160.2.493-498.1984

Genes

Locus	Cultural Requirements	Link Group	Type
nmr-1	VR. Between am (3 to 7%) and gln-1 (4 to 10%) (1079).Synthesis of nitrate reductase is derepressed on ammonium, glutamate, or glutamine. Hypostatic to nit-2 and nit-4. Prototrophic. Isolated and scored by sensitivity to chlorate in the presence of glutamine. (295, 1079) MS5 is a possible allele. MS5 is unmapped and is not allelic with nit-2 or nit-3 on the basis of two wild-type ascospores from poorly fertile crosses in each case. MS5 is derepressed on glutamine. Levels of nitrate reductase, nitrite reductase, histidase, and acetamidase are elevated in the presence of glutamine and the respective enzyme inducer. Prototrophic. Scored the same as the mutant nmr-1 and checked by assaying for nonrepressibility of nitrate reductase by glutamine. (838; G.J. Sorger, personal communication)	VR	B

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