FGSC #4590

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Mutant Type

Genus: N

reporting_genes: uvs-3

species: Neurospora crassa

allele: FK016

stock: M1195a

glasgow:

mutagen: UV

Depositor: EK

Link Group: IVL

MT: a

Species No: 10

gene_back: SL8

oppmt: 4589

trans:

ref1: Kafer, 1983. Genetics 105:19, https://doi.org/10.1093/genetics/105.1.19

ref2: Kafer & Witchell 1984. Biochem. Genet. 22(5/6):403, https://doi.org/10.1007/bf00484512

site:

country:

ksudc_link: https://digital.lib.k-state.edu/item/neurospora-crassa/fgsc-4590

ksudc_link_html: https://digital.lib.k-state.edu/item/neurospora-crassa/fgsc-4590 ↗

Genes

Locus	Cultural Requirements	Link Group	Type
uvs-3	VL. Linked to cys-10 (3 to 7%), probably to the left (538, 932).Allele ALS11 is sensitive to UV (273, 932, 933), ionizing radiation (537, 933, 940), methyl methane sulfonate (536), nitrosoguanidine (509, 933), mitomycin C (195, 537), histidine (932), and 4-nitroquinoline 1-oxide and ICR-170 (509). Reverts spontaneously (932). No UV-induced mutation (273). For mutation induction by other agents, see references 509 and 940. Increased spontaneous mutation (275, 537). Dimer excision delayed and at reduced rate (1164). Defective photoreactivation in vivo, but photoreactivation enzyme functions in vitro (934). Defective in extracellular nuclease, giving reduced halo around colonies on DNA agar (538). Apparently deficient in proteolytic conversion of nuclease precursor to active intra- and extracellular deoxyribonucleases, but this effect could be indirect (360). Increased stability of CPS(Pyr) and ACT activities in vitro also suggests that protease activity may be reduced in uvs-3 mutants (882). Causes increased duplication instability (mitotic recombination or deletion or both) (932). Conidial viability is low (275, 932). Double mutant upr-1;uvs-3 is much more sensitive to UV than is either single mutant (1095). Double mutant uvs-3;uvs-6 is inviable (506). Homozygous barren, with block before karyogamy (860, 932). Shows high level of repair of genetic damage without induction in rescuing a heterokaryotic component that carries potentially lethal mutagen-induced damage (1022). Probable allele FKO16, isolated as halo mutation nuh-4, resembles uvs-3 allele ALS11 but is less extreme in some properties, e.g., it shows better conidial survival (538; see reference 537).	VL	B