FGSC #4475

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Reporting Genes: un-2;thi-4;lys-1

Species: crassa

Allele: see 4488

Depositor: RLM

Mating Type: a

Species Number: 10

ref1: Metzenberg, et al NN 31:35, https://doi.org/10.4148/1941-4765.1608

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
lys-1	V. Right of caf-1 (4 to 14%). Left of cyt-9 (5%) and at (1 to 20%) (817; K.S. Hsu, personal communication; PB). (403)Uses lysine, alpha-aminoadipic acid, or epsilon-hydroxynorleucine (alpha-amino-epsilon-hydroxycaproic acid) (399, 400, 684, 1087). Accumulates homocitrate on limiting lysine concentrations (464) (Fig. 16). Fine structure and complementation between alleles (8). Initial allele: 33933.	V	B
thi-4	III. Linked to acr-2, spg, and sc (< 1%). Left of pro-1(3%) and ace-2 (4 to 9%) (812). (482). Requires thiamine (301). Anomalous in condensation of the pyrimidine and thiazole precursors. Very leaky (301). A probable allele called "thi-lo" greatly increases the thiamine requirement of thi-1 strains and decreases the ability to synthesize thiamine from pyrimidine and thiazole. thi-lo strains have no detectable nutritional requirement in the absence of thi-1. thi-lo failed to recombine with thi-4 among 55 scorable progeny (301).	III	B
un-2	IR. Between the T(AR173) left breakpoint and T(AR190); hence, right of the centromere and arg-3 (1 to 2%) and left of his-2 (1%). Included in duplications from T(AR173) but not from T(AR190) (808). (482). Unknown function. Heat sensitive (482). Growth at 25°C but not at 39°C. Scorable but leaky at 34°C. Formerly called un(46006).	IR	B