FGSC #4457

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Reporting Genes: arg-5;thi-4;pyr-1;lys-1

Species: crassa

Allele: see 4488

Depositor: RLM

Mating Type: A

Species Number: 10

ref1: Metzenberg, et al NN 31:35, https://doi.org/10.4148/1941-4765.1608

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
arg-5	Uses ornithine, citrulline or arginine.	IIR	B
lys-1	V. Right of caf-1 (4 to 14%). Left of cyt-9 (5%) and at (1 to 20%) (817; K.S. Hsu, personal communication; PB). (403)Uses lysine, alpha-aminoadipic acid, or epsilon-hydroxynorleucine (alpha-amino-epsilon-hydroxycaproic acid) (399, 400, 684, 1087). Accumulates homocitrate on limiting lysine concentrations (464) (Fig. 16). Fine structure and complementation between alleles (8). Initial allele: 33933.	V	B
pyr-1	IVR. Right of psi (4%) and T(ALS159) (808, PB). Left of pdx-1 (<1 to 10%) (692). (482) Requires uracil or other pyrimidine. Lacks dihydroorotate dehydrogenase activity (133, 134) (Fig. 20). All ascospores from pyr-1 x pyr-1crosses are white if cross is on medium containing 0.1 mg of uracil per ml; they are black if the cross is on medium containing 1.0 mg/ml (632).	IVR	B
thi-4	III. Linked to acr-2, spg, and sc (< 1%). Left of pro-1(3%) and ace-2 (4 to 9%) (812). (482). Requires thiamine (301). Anomalous in condensation of the pyrimidine and thiazole precursors. Very leaky (301). A probable allele called "thi-lo" greatly increases the thiamine requirement of thi-1 strains and decreases the ability to synthesize thiamine from pyrimidine and thiazole. thi-lo strains have no detectable nutritional requirement in the absence of thi-1. thi-lo failed to recombine with thi-4 among 55 scorable progeny (301).	III	B