FGSC #4442

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Reporting Genes: (ufa(P73B118)A + a[m1] ad-3B cyh-1)

Species: crassa

Allele: P73B118 + 1 B114 KH52

Alternate Strain Number: 507

Depositor: DDP

Linkage Group: IVR

Mating Type: A

Species Number: 10

Opposite Mating Type: 4443

ref1: Griffths & DeLange 1978 Genetics 88:239-254, https://doi.org/10.1093/genetics/88.2.239

ref2: https://doi.org/10.1128/jb.130.3.1144-1148.1977

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
ad-3B	IR. Between ad-3A (0.1 to 0.7%) and nic-2 (3%) (271). (482) Uses adenine or hypoxanthine (682). Blocked in interconversion of AIR to CAIR (348) (Fig. 8). Produces purple pigment, permitting direct visual selection (276, 682). Pigment is secreted with low concentrations of adenine (e.g., 0.1 mM), not with high concentrations (2 mM) (276, 682, 785). Pigment production used to assess effect of histidine and tryptophan on purine nucleotide synthesis (786). Reduced interallelic fertility (264, 407). Complementation maps (268, 274). Relation of mutagens to complementation patterns (269). Mutants with non-polarized complementation patterns on the right side of the complementation map grow on minimal medium if supplied with CO2; other mutants do not respond to CO2, (270). Used extensively for mutagenesis (see ad-3A). Rearrangement T(I- >III)Y112M4i ad-3B, which has a breakpoint inseparable from ad-3B, was the first insertional translocation to be reported for fungi (266). Allele 7-017-0137 shows "fixed instability," mutating to an unstable prototrophic allele (41). Alleles 2-17-126, 12-21-28, and numerous others are supersuppressible (408, 749, 955). Called complementation group B.	IR	B
am1			B
cyh-1	IR. Right of nit-1 (6%). Left of T(STL76) and al-2 (8 to 13%) (496, 797, 808).Resistant to cycloheximide (496, 748). Resistance is recessive in duplications (1090). Dominance reported in forced heterokaryons (496, 748) may have been due to skewed nuclear ratios (1090). Protein synthesis on ribosomes of the mutant cyh-1 proceeds in the presence of cycloheximide in a cell-free system (834). Readily scored on slants with 10 µg of cycloheximide per ml autoclaved in the medium. Excellent as a marker and valuable for selecting somatic recombinants or deletions in heterozygous duplications (748, 1091). Used to show that the cycloheximide-induced phase shift of the circadian clock involves protein synthesis (738). Called act-1: actidione resistant-1.	IR	B
ufa(P73B118)A	IV or V. Linked to inl in a cross with alcoy (944). Data do not distinguish linkage group because T(IVR;VR)R2355 was present.Requirement satisfied by C16 or C18 fatty acids having a double bond in cis at either the Delta9 or Delta11 position, by C16 fatty acids with double bond in trans at Delta9, or by fatty acids with multiple cis double bonds interrupted by methylene bridges (944, 945). Reverts readily, and revertants overgrow culture if grown on suboptimal supplement (S. Brody, personal communication). Tween 80 (0.1%) is satisfactory for maintenance. Stock viability is better on Tween 80 than on fatty acids suspended in detergent Tergitol Nonidet P-40 (944). For biosynthetic pathway see reference 944.	IV or V	B