FGSC #4303

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Reporting Genes: acr-3 un-16

Species: crassa

Allele: KH14(r) T42M69(t)

Alternate Strain Number: 1710

Depositor: DDP

Linkage Group: IL IL

Mating Type: a

Species Number: 10

Genetic Background: SL

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
un-16	IL. Right of mt (<1%). Left of ta (1%) and acr-3(<3%). Closest flanking marker right of the mating type locus (818, PB). Unknown function. Heat sensitive, 34°C versus 25°C (507). Allele T42M69 is called 16 in reference 507.	IL	B
acr-3	IL. Between un-16 (1 to 5%) and suc (1 to 5%). Probably right of ta (816; PB). (498)Resistant to acriflavine and to malachite green (three alleles tested). Not resistant to 3-aminotriazole. Resistance is probably dominant (heterokaryon tests) (498). Scoring is clear-cut with uniform inocula of appropriate size. False-negative or false-positive scoring may result if test inocula are too small or too large. May show delayed resistance: read tests at 2 and 4 days, 34°C. Use acriflavine at 10 µg /ml in minimal agar at 34°C (816) and malachite green at 2 µg /ml (498).	IL	B